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Product name
Anti-Zebrafish Intestinal Epithelium Basolateral Pole Cells antibody [2H9]
Description
Mouse monoclonal [2H9] to Zebrafish Intestinal Epithelium Basolateral Pole Cells
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Zebrafish
Immunogen
Tissue / cell preparation (Zebrafish): Lysate of zebrafish intestine.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Primary antibody notes
The transparency of the juvenile zebrafish and it's genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. Antibody 2H9 recognises a membrane-associated epitope on the gut epithelial cells, concentrated basolaterally. In one month old zebrafish it appears especially prominent in the basl parts of the elongated cells of the villi. This antibody is a useful marker of apicobasal polarity in the epithelium.
Clonality
Monoclonal
Clone number
2H9
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ICC/IF
Application notes
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This antibody is also suitable for use in histology.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Anti-Zebrafish Intestinal Epithelium Basolateral Pole Cells antibody [2H9] (ab71504)
This product has been referenced in:
- Crosnier C et al. Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine. Development 132:1093-104 (2005).Read more (PubMed: 15689380) »
See 1 publication for this product
Publishing research using ab71504? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"