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Product name
Anti-Zebrafish Posterior Intestinal Goblet Cells antibody [3G12]
Description
Mouse monoclonal [3G12] to Zebrafish Posterior Intestinal Goblet Cells
Specificity
The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. Antibody 3G12 marks goblet cells specifically. It labels both the mucus compartment and other parts of the cytoplasm. On Western blots, 3G12 stains diffuse bands, consistent with variably glycosylated antigen(s). 3G12 labelled cells are concentrated in the posterior gut. 3G12 is a marker for mucus secreting cells in the posterior gut of zebrafish.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Zebrafish
Immunogen
Tissue / cell preparation (Zebrafish): Lysate of Zebrafish gut intestine.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
3G12
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Research areas
Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ICC/IF
Application notes
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This antibody is also suitable for use in histology.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Anti-Zebrafish Posterior Intestinal Goblet Cells antibody [3G12] (ab71508)
This product has been referenced in:
- Crosnier C et al. Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine. Development 132:1093-104 (2005).Read more (PubMed: 15689380) »
See 1 publication for this product
Publishing research using ab71508? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"