You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Zeta Opioid Receptor protein (Tagged-His Tag) (ab92143)

Submit an Abreview Submit a Question

Overview

  • Product nameZeta Opioid Receptor protein (Tagged-His Tag)See all Zeta Opioid Receptor proteins and peptides ...
  • Protein descriptionSynthetic peptide, corresponding to amino acids 539-677 of Human Zeta Opioid Receptor, with N terminal His tag; 139 amino acids, 43kDa (13kDa predicted from sequence). UniProt ID = Q9NZT2
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 117 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesSETPGPRPAGPAGDEPAESPSETPGPRPAGPAGDEPAESP SE TPGPSPAGPTRDEPAESPSETPGPRPAGPAGDEPAE SPSETP GPRPAGPAGDEPAESPSETPGPSPAGPTRDEP AKAGEAAELQ DAEVESSAKSGKP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92143 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        7 60 proteinHGNC:15768Met enkephalin receptor
        OGFROGFR_HUMANOpioid growth factor receptorProtein 7-60Zeta type opioid receptorZeta-type opioid receptor
      see all
  • FunctionReceptor for opioid growth factor (OGF), also known as Met-enkephalin. Seems to be involved in growth regulation.
  • Tissue specificityHighly expressed in the heart and liver, moderately in skeletal muscle and kidney and to a lesser extent in brain and pancreas. Expressed in fetal tissues including liver and kidney.
  • Sequence similaritiesBelongs to the opioid growth factor receptor family.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. The OGF/OGFR complex is probably translocated to the nucleus.

    • Target information above from: UniProt accession Q9NZT2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Zeta Opioid Receptor protein (Tagged-His Tag) images

    • SDS-PAGE - Zeta Opioid Receptor protein (Tagged-His Tag) (ab92143)
      SDS-PAGE - Zeta Opioid Receptor protein (Tagged-His Tag) (ab92143)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Zeta Opioid Receptor protein (Tagged-His Tag) (ab92143)

    ab92143 has not yet been referenced specifically in any publications.

    Publishing research using ab92143 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab92143 Submit a Question for ab92143

    There are currently no Abreviews or Questions for ab92143.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"