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Western blot - Zfp281 antibody [148C4a] (ab53574)
Western blot - Zfp281 antibody [148C4a] (ab53574)
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Product Name 

Zfp281 antibody [148C4a]

 

See all Zfp281 antibodies (5)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [148C4a] to Zfp281

Immunogen 

Recombinant human Zfp281

Reacts with 
(species key)

Hu

Tested applications 
(see key)

Dot, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 35 kDa (predicted molecular weight: 97 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Stem Cells >> Embryonic Stem Cells >> Intracellular
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

This zinc finger protein is involved in transcriptional regulation. It represses the transcription of a number of genes including gastrin and ornithine decarboxylase. It binds to the G-rich box in the enhancer region of these genes and may also complex with Oct4.

 

Alternative names Zfp281 antibody [148C4a] (ab53574)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

23528    (Human)

SwissProt

    

Q9Y2X9    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

148C4a

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4)

Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS

Purification notes 

ab53574 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Zfp281 antibody [148C4a] is on this datasheet. But please do contact us if you would like any reassurance!


See below for Zfp281 antibody [148C4a] images, references, products related to ab53574 and other tools.

 

Zfp281 antibody [148C4a] images:

Product image  Western blot - Zfp281 antibody [148C4a] (ab53574)

Western blot - Zfp281 antibody [148C4a] (ab53574)

Zfp281 antibody [148C4a] (ab53574) + immunised recombinant protein

Predicted band size : 97 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to Zfp281

 

Zfp281 antibody [148C4a] - more information

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Price and availability of products related to Zfp281 antibody [148C4a]

 

Zfp281 antibody [148C4a] - related products:

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