![Western blot - Zfp281 antibody [148C4a] (ab53574) image](#ab53574.gif)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Zfp281 antibody [148C4a]
See all Zfp281 products (6) ...
Description
Mouse monoclonal [148C4a] to Zfp281
Tested applications
WB, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant human Zfp281
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4)
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab53574 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Clonality
Monoclonal
Clone number
148C4a
Isotype
IgG1
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Western blot - Zfp281 antibody [148C4a] (ab53574)
![Western blot - Zfp281 antibody [148C4a] (ab53574) image](/ps/datasheet/Images/53/ab53574/ab53574.gif)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 35 kDa (predicted molecular weight: 97 kDa).
Dot: Use at an assay dependent dilution.
Target
Function
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes.
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9Y2X9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- GC box binding zinc finger protein 1 antibody
- GC-box-binding zinc finger protein 1 antibody
- GZP1 antibody
see all
Database links
- Entrez Gene: 23528 Human
- SwissProt: Q9Y2X9 Human
- Unigene: 59757 Human
Anti-Zfp281 antibody [148C4a] images:
Western blot - Zfp281 antibody [148C4a] (ab53574)
Anti-Zfp281 antibody [148C4a] (ab53574) + immunised recombinant protein
Predicted band size : 97 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
References for Anti-Zfp281 antibody [148C4a] (ab53574)
ab53574 has not yet been referenced specifically in any publications.
Publishing research using ab53574? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"