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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Zfp281 antibody
See all Zfp281 products (6) ...
Description
Rabbit polyclonal to Zfp281
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human) from centre Zfp281 conjugated to KLH
Positive control
293 cell lysate transfected with Zfp281
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Western blot - Zfp281 antibody (ab75733)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100.Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Function
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes.
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9Y2X9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- GC box binding zinc finger protein 1 antibody
- GC-box-binding zinc finger protein 1 antibody
- GZP1 antibody
see all
Database links
- Entrez Gene: 23528 Human
- SwissProt: Q9Y2X9 Human
- Unigene: 59757 Human
Anti-Zfp281 antibody images:
Western blot - Zfp281 antibody (ab75733)
All lanes : Anti-Zfp281 antibody (ab75733) at 1/50 dilution
Lane 1 : Non transfected 293 cell lysate
Lane 2 : 293 cell lysate transfected with Zfp281
Lysates/proteins at 2 µg per lane.
Predicted band size : 97 kDa
Observed band size : 97 kDa
References for Anti-Zfp281 antibody (ab75733)
ab75733 has not yet been referenced specifically in any publications.
Publishing research using ab75733? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"