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Western blot - Zfp281 antibody (ab75733)

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Product Name
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Zfp281 antibody
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See all Zfp281 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Zfp281
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Immunogen
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Synthetic peptide (Human) from centre Zfp281 conjugated to KLH
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: 1/1000. WB: 1/50 - 1/100. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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293 cell lysate transfected with Zfp281
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules Stem Cells >> Embryonic Stem Cells >> Intracellular Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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This zinc finger protein is involved in transcriptional regulation. It represses the transcription of a number of genes including gastrin and ornithine decarboxylase. It binds to the G-rich box in the enhancer region of these genes and may also complex with Oct4.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 23528 (Human)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Ammonium Sulphate Precipitation
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Storage buffer
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Preservative: 0.09% Sodium Azide Constituents: PBS Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Zfp281 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Zfp281 antibody images, references, products related to ab75733 and other tools.
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Zfp281 antibody images:
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Western blot - Zfp281 antibody (ab75733)
All lanes : Zfp281 antibody (ab75733) at 1/50 dilution
Lane 1 : Non transfected 293 cell lysate Lane 2 : 293 cell lysate transfected with Zfp281
Lysates/proteins at 2 µg per lane.
Predicted band size : 97 kDa Observed band size : 97 kDa
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Search PubMed (MEDLINE) for references to Zfp281
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Zfp281 antibody - more information
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Zfp281 antibody - related products:
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Zfp281 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Zfp281 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"