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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-Zhangfei antibody
See all Zhangfei products (3) ...
Description
Rabbit polyclonal to Zhangfei
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Zhangfei.
(Peptide available as ab28713.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: 293T Embryonic Kidney cell line (Human), HepG2 (Human hepatocellular liver carcinoma cell line), MCF7 (Human breast adenocarcinoma cell line), HeLa (Human epithelial carcinoma cell line), PC3 (Human prostate cancer cell line), NIH 3T3 (Mouse embryonic fibroblast cell line). This antibody also gave a positive signal in the following tissue lysates: Human Heart, Human Brain, Human Kidney, Human Liver.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).
Target
Function
Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells.
Tissue specificity
In adults, expressed most abundantly in heart, liver and skeletal muscle, moderately abundant in kidney and pancreas, and barely detectable in lung. In fetal tissues, expressed most abundantly in kidney and very low amounts in heart, lung and liver.
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Cellular localization
Nucleus. Colocalizes at punctate nuclear structures with CREB3 and HCFC1.
Target information above from: UniProt accessionQ9NS37
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 6330417B10Rik antibody
- AI225749 antibody
- AI606244 antibody
see all
Database links
- Entrez Gene: 58487 Human
- Entrez Gene: 233490 Mouse
- Omim: 606444 Human
- SwissProt: Q9NS37 Human
- SwissProt: Q91ZR3 Mouse
- Unigene: 535319 Human
- Unigene: 714910 Human
- Unigene: 723938 Human
see all
Anti-Zhangfei antibody images:
Western blot - Anti-Zhangfei antibody (ab28700)
All lanes : Anti-Zhangfei antibody (ab28700) at 1 µg/ml
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lane 5 : 293T Embryonic Kidney cell line (Human) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 9 : PC3 (Human prostate cancer cell line) Whole Cell Lysate (ab3954)
Lane 10 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes
In the literature Zhangfei is also referred to as SMILE (SHP-interacting leucine zipper protein). The SMILE gene generates two isoforms, SMILE-L (long isoform of SMILE, 50–60 kDa) and SMILE-S (short isoform of SMILE, 40 kDa), and ab28700 predominantly recognizes the SMILE-L form.
Immunocytochemistry/ Immunofluorescence - Zhangfei antibody (ab28700)
ICC/IF image of ab28700 stained human HEK293 cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab28700, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
References for Anti-Zhangfei antibody (ab28700)
This product has been referenced in:
- Misra Jet al. Curcumin Differentially Regulates Endoplasmic Reticulum Stress through Transcriptional Corepressor SMILE (Small Heterodimer Partner-interacting Leucine Zipper Protein)-mediated Inhibition of CREBH (cAMP Responsive Element-binding Protein H). J Biol Chem 286:41972-84 (2011).Read more (PubMed: 21994947) »
- Xie YBet al. Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation. J Biol Chem 284:28762-74 (2009). WB; Human.Read more (PubMed: 19690166) »
See all 3 publications for this product
Publishing research using ab28700? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"