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Western blot - Zhangfei antibody (ab28700)
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Immunocytochemistry/ Immunofluorescence - Zhangfei antibody (ab28700)
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Product Name
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Zhangfei antibody
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See all Zhangfei antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Zhangfei
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Zhangfei. (Peptide available as ab28713.)
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Predicted to react
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Hu, Ms
(Due to sequence homology)
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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Zhangfei strongly activates transcription when bound to HCFC1. It suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. It also suppresses the HCFC1 dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. It can down-regulate expression of some cellular genes in ZF expressing cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.690 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Zhangfei antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Zhangfei antibody images, references, products related to ab28700 and other tools.
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Zhangfei antibody images:
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 Western blot - Zhangfei antibody (ab28700)
All lanes : Zhangfei antibody (ab28700) at 1 µg/ml
Lane 1 : Heart (Human) Tissue Lysate
Lane 2 : Brain (Human) Tissue Lysate
Lane 3 : Kidney (Human) Tissue Lysate
Lane 4 : Liver (Human) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
We are unsure about the expression of the Zhangfei protein so cannot explain the bands observed in Western Blot. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunocytochemistry/ Immunofluorescence - Zhangfei antibody (ab28700)
ICC/IF image of ab28700 stained human HEK293 cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab28700, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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References for Zhangfei antibody (ab28700)
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This product has been used in:
Xie YB et al. Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation. J Biol Chem 284:28762-74 (2009). WB; Human. PubMed: 19690166
If you publish research using ab28700 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Zhangfei
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Zhangfei antibody - more information
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Customer reviews (feedback) regarding Zhangfei antibody |
Customer FAQs regarding Zhangfei antibody |
Protocols for Zhangfei antibody |
Price and availability of products related to Zhangfei antibody |
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Zhangfei antibody - related products:
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Zhangfei antibody - other tools:
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Contact Abcam with a Technical Enquiry about Zhangfei antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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