Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Zic1 antibody
See all Zic1 products (3) ...
Description
Rabbit polyclonal to Zic1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Zic1.
(Peptide available as ab25748.)
Positive control
This antibody gave a positive signal in human brain tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neural Signal Transduction
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
-
Western blot - Zic1 antibody (ab24204)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
Application notes
Is unsuitable for ,IHC-FoFr or IHC-P.
Target
Function
Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.
Tissue specificity
CNS. A high level expression is seen in the cerebellum. Detected in the nuclei of the cerebellar granule cell lineage from the progenitor cells of the external germinal layer to the postmigrated cells of the internal granular layer. Detected in medulloblastoma (26/29 cases), but not present in all other tumors examined.
Sequence similarities
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
Domain
The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
Cellular localization
Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression.
Target information above from: UniProt accessionQ15915
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Odd paired homolog Drosophila antibody
- Zic 1 antibody
- ZIC antibody
see all
Database links
- Entrez Gene: 7545 Human
- Entrez Gene: 22771 Mouse
- Entrez Gene: 64618 Rat
- Omim: 600470 Human
- SwissProt: Q15915 Human
- SwissProt: P46684 Mouse
- Unigene: 41154 Human
- Unigene: 598590 Human
see all
Anti-Zic1 antibody images:
Western blot - Zic1 antibody (ab24204)
Anti-Zic1 antibody (ab24204) at 1 µg/ml + Human Brain Tissue Lysate at 20 µg
Performed under reducing conditions.
Predicted band size : 48 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa (possible cleavage fragment,cross reactivity).
References for Anti-Zic1 antibody (ab24204)
This product has been referenced in:
- Arora Aet al. Inhibition of translation in living eukaryotic cells by an RNA G-quadruplex motif. RNA 14:1290-6 (2008).Read more (PubMed: 18515550) »
See 1 publication for this product
Publishing research using ab24204? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"