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Rabbit polyclonal to A RAF (phospho S299)
ab112053 detects endogenous levels of A RAF only when phosphorylated at serine 299.
Predicted to work with:
Synthetic phosphopeptide derived from Human A RAF around the phosphorylation site of serine 299 (R-D-S
Human colon carcinoma tissue.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide Constituents: 0.88% Sodium chloride, 50% Glycerol, 49% PBS
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Immunogen affinity purified
ab112053 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Predominantly in urogenital tissues.
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 RBD (Ras-binding) domain.
Information by UniProt
A raf 1 antibody
A Raf proto oncogene serine/threonine protein kinase antibody
ARAF 1 antibody
has not yet been referenced specifically in any publications.
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