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Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
Function Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Tissue specificity Predominantly in urogenital tissues.
Sequence similarities Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 RBD (Ras-binding) domain.
Information by UniProt
A raf 1 antibody
A Raf proto oncogene serine/threonine protein kinase antibody
ARAF 1 antibody
Anti-A RAF (phospho Y301 + Y302) antibody images
Western blot - A RAF (phospho Y301 + Y302) antibody (ab59439)
All lanes : Anti-A RAF (phospho Y301 + Y302) antibody (ab59439) at 1/500 dilution Lane 1 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins) Lane 2 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins) with phosphopeptide Predicted band size : 68 kDa Observed band size : 68 kDa
References for Anti-A RAF (phospho Y301 + Y302) antibody (ab59439)
has not yet been referenced specifically in any publications.
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