Anti-A RAF (phospho Y301 + Y302) antibody (ab59439)

Overview

  • Product nameAnti-A RAF (phospho Y301 + Y302) antibody
    See all A RAF primary antibodies
  • Description
    Rabbit polyclonal to A RAF (phospho Y301 + Y302)
  • SpecificityDetects endogenous levels of A RAF only when phosphorylated at tyrosine 301/tyrosine 302.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide (Human) from around the phosphorylation site of tyrosine 301/tyrosine 302 (GYYPWE) of A RAF.

  • Positive control
    • HeLa cell extract treated with PMA (125ng/ml, 30mins).
  • General notesPhosphorylation sites: Human: Tyr301/Tyr302; Mouse: Tyr299/Tyr300; Rat: Tyr299/Tyr300

Properties

Applications

Our Abpromise guarantee covers the use of ab59439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
ELISA 1/20000.

Target

  • FunctionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus.
  • Tissue specificityPredominantly in urogenital tissues.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • A raf 1 antibody
    • A Raf proto oncogene serine/threonine protein kinase antibody
    • ARAF 1 antibody
    • Araf antibody
    • ARaf proto oncogene serine/threonine protein kinase antibody
    • ARAF_HUMAN antibody
    • ARAF1 antibody
    • Oncogene Araf1 antibody
    • Oncogene PKS2 antibody
    • PKS 2 antibody
    • PKS antibody
    • PKS2 antibody
    • Proto oncogene Pks antibody
    • Proto-oncogene A-Raf antibody
    • Proto-oncogene A-Raf-1 antibody
    • Proto-oncogene Pks antibody
    • RAFA 1 antibody
    • RAFA1 antibody
    • Ras binding protein DA Raf antibody
    • Serine/threonine-protein kinase A-Raf antibody
    • v raf murine sarcoma 3611 viral oncogene homolog antibody
    • v raf murine sarcoma 3611 viral oncogene homolog 1 antibody
    • v raf oncogene homolog 1 (murine sarcoma 3611 virus) antibody
    see all

Anti-A RAF (phospho Y301 + Y302) antibody images

  • All lanes : Anti-A RAF (phospho Y301 + Y302) antibody (ab59439) at 1/500 dilution

    Lane 1 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins)
    Lane 2 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins) with phosphopeptide


    Predicted band size : 68 kDa
    Observed band size : 68 kDa

References for Anti-A RAF (phospho Y301 + Y302) antibody (ab59439)

ab59439 has not yet been referenced specifically in any publications.

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