Overview

  • Product nameAnti-A1CF antibodySee all A1CF primary antibodies ...
  • Description
    Rabbit polyclonal to A1CF
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (EAVCLGTCPE PEASMSTAIP GLKKGNNALQ SIILQTLLEK ENGQRKYGGP) of human A1CF (NP_620311).

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

Target

  • FunctionEssential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay.
  • Tissue specificityWidely expressed with highest levels in brain, liver, pancreas, colon and spleen.
  • Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
  • DomainThe RRM domains are necessary but not sufficient for binding to APOB mRNA. Additional residues in the pre-RRM and C-terminal regions are required for RNA-binding and for complementing APOBEC1 activity.
  • Cellular localizationNucleus. Endoplasmic reticulum. Cytoplasm. Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1.
  • Target information above from: UniProt accession Q9NQ94 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • A1CF antibody
    • A1CF_HUMAN antibody
    • ACF antibody
    • ACF64 antibody
    • ACF65 antibody
    • Apo B RNA editing protein antibody
    • Apobec 1 complementation factor antibody
    • Apobec 1 complementation factor (ACF) (ASP) antibody
    • APOBEC 1 stimulating protein antibody
    • APOBEC1 complementation factor antibody
    • APOBEC1 stimulating protein antibody
    • APOBEC1-stimulating protein antibody
    • APOBEC1CF antibody
    • ASP antibody
    • MGC163391 antibody
    • RP11-564C4.2 antibody
    see all

Anti-A1CF antibody images

  • Anti-A1CF antibody (ab83276) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 65 kDa

References for Anti-A1CF antibody (ab83276)

ab83276 has not yet been referenced specifically in any publications.

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