Overview

  • Product nameAnti-A2B5 antibody [105]
    See all A2B5 primary antibodies
  • Description
    Mouse monoclonal [105] to A2B5
  • Tested applicationsFlow Cyt, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Embryonic chicken retinal cells.

  • Positive control
    • Type II astrocytes, human neural progenitors.

Properties

Applications

Our Abpromise guarantee covers the use of ab53521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
IHC-Fr Use at an assay dependent dilution.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Target

  • RelevanceA2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells.
  • Cellular localizationCell surface

Anti-A2B5 antibody [105] images

  • ICC/IF image of ab53521 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53521, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgM (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • A2B5 antibody [105] - Stem Cell Marker (ab53521) used in immunocytochemical detection on Cor1 neuronal stem cells. Cor1 neuronal stem cells were fixed in formaldehyde, permeabilized, blocked in 1% BSA for 30 mins at RT. ab53521 was incubated at 1/500 for 16 hours in TBS/BSA/azide/0.5% Triton. Secondary Antibody:anti mouse IgM conjugated: to Alexa Fluor® 488 (1/1000).

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab53521 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53521, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-A2B5 antibody [105] (ab53521)

This product has been referenced in:
  • Chen LP  et al. Regulation of Olig2 during astroglial differentiation in the subventricular zone of a cuprizone-induced demyelination mouse model. Neuroscience 221:96-107 (2012). Read more (PubMed: 22771621) »
  • Paintlia MK  et al. Modulation of peroxisome proliferator-activated receptor-alpha activity by N-acetyl cysteine attenuates inhibition of oligodendrocyte development in lipopolysaccharide stimulated mixed glial cultures. J Neurochem 105:956-70 (2008). Read more (PubMed: 18205750) »

See all 25 Publications for this product

Product Wall

I have review the availible literature regarding the A2B5 epitope and have asked the lab to search as well. Unfortunately, we have found no instances recording the mRNA sequence for this target. A2B5 seems to have first been recognized in 1979 in by Ei...

Read More

Thank you for your inquiry.

Yes, it does appear these are the same product as they have the same clone number.

I hope this information helps. Please contact us with any other questions.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

Read More

I am sorry this antibody is still not working for you. I will need the Abcam reference number for the order (six digits) or a PO number, or the date of the order and your institution. Did you have an antibody in mind that you would like to replace it w...

Read More

Thank you for bringing this issue to our attention. As I mentioned, if the PC12 stain does not work and you have confirmed that the secondary is appropriate (and that it is still fluorescent), I will replace the antibody. There is a chance that a singl...

Read More

I am very pleased to hear you would like to accept our offer and test ab66253 and ab53521 in IHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this c...

Read More

Thank you for contacting Abcam. Attached please find the Certificate of Compliance documents you requested. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 22 2010

Thank you for your patience. The lab has finally isotyped the product and confirmed that it is a kappa isotype. Therefore, the suitable isotype control for this product is: ab18401 Mouse IgM, kappa monoclonal [MM-30] - isotype control Ple...

Read More

Thank you for your enquiry. Since the primary antibody is mouse IgM, you would need to use a secondary antibody that reacts with mouse IgM (anti-Mouse IgM or anti-Mouse IgG (Fab)). I am afraid that a mouse IgG might not suitable. I hope this in...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"