Anti-A2BP1 / Fox1 antibody (ab83574)

Overview

  • Product nameAnti-A2BP1 / Fox1 antibody
    See all A2BP1 / Fox1 primary antibodies
  • Description
    Rabbit polyclonal to A2BP1 / Fox1
  • Tested applicationsWB, ELISA, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide from within the N terminal amino acids 1-50: MNCEREQLRG NQEAAAAPDT MAQPYASAQF APPQNGIPAE YTAPHPHPAP, of Human A2BP1/ Fox1 (NP_001135805)

  • Positive control
    • MCF7 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

IHC-FoFr Use at an assay dependent concentration.

Target

  • FunctionRNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
  • Tissue specificityPredominantly expressed in muscle and brain.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2BP1 antibody
    • A2 BP1 antibody
    • A2BP 1 antibody
    • A2BP antibody
    • A2BP1 antibody
    • Ataxin 2 binding protein 1 antibody
    • Ataxin-2-binding protein 1 antibody
    • Ataxin2 binding protein 1 antibody
    • FOX 1 antibody
    • Fox-1 homolog A antibody
    • fox-1-like RNA-binding protein 1 antibody
    • FOX1 antibody
    • Hexaribonucleotide binding protein 1 antibody
    • Hexaribonucleotide-binding protein 1 antibody
    • HRNBP 1 antibody
    • HRNBP1 antibody
    • RBFOX1 antibody
    • RFOX1_HUMAN antibody
    • RNA binding protein fox-1 homolog 1 antibody
    • RNA binding protein fox-1 homolog 1, C. elegans, homolog of, 1 antibody
    • RNA binding protein, fox-1 homolog (C. elegans) 1 antibody
    see all

Anti-A2BP1 / Fox1 antibody images

  • Anti-A2BP1 / Fox1 antibody (ab83574) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References for Anti-A2BP1 / Fox1 antibody (ab83574)

ab83574 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

Under our Abpromise, which is valid for 6 months after delivery, we guarantee ab83574 to work in western blot, ELISA & IHC-FoFr on mouse and human tissue. We have gone through a number of different lots of t...

Read More

Thank you for your enquiry regarding our two antibodies (ab83574 and ab94581). Generally, brain (CNS) cells express Fox1 at high levels particularly in the following region cerebral cortex, hippocampus and lateral ventricle. As the datasheets indic...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Coronal Brain)
Specification Coronal Brain
Fixative Paraformaldehyde
Permeabilization Yes - PBS + 0.1% Tween20
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"