Anti-A2BP1 / Fox1 antibody (ab94581)

Overview

  • Product nameAnti-A2BP1 / Fox1 antibodySee all A2BP1 / Fox1 primary antibodies ...
  • Description
    Rabbit polyclonal to A2BP1 / Fox1
  • Tested applicationsICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Dog, Monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human A2BP1/ Fox1.

  • Positive control
    • This antibody gave a positive signal in Mouse Forebrain Tissue and MCF7 Whole Cell lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human skeletal muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab94581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionRNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
  • Tissue specificityPredominantly expressed in muscle and brain.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2BP1 antibody
    • A2 BP1 antibody
    • A2BP 1 antibody
    • A2BP antibody
    • A2BP1 antibody
    • Ataxin 2 binding protein 1 antibody
    • Ataxin-2-binding protein 1 antibody
    • Ataxin2 binding protein 1 antibody
    • FOX 1 antibody
    • Fox-1 homolog A antibody
    • fox-1-like RNA-binding protein 1 antibody
    • FOX1 antibody
    • Hexaribonucleotide binding protein 1 antibody
    • Hexaribonucleotide-binding protein 1 antibody
    • HRNBP 1 antibody
    • HRNBP1 antibody
    • RBFOX1 antibody
    • RFOX1_HUMAN antibody
    • RNA binding protein fox-1 homolog 1 antibody
    • RNA binding protein fox-1 homolog 1, C. elegans, homolog of, 1 antibody
    • RNA binding protein, fox-1 homolog (C. elegans) 1 antibody
    see all

Anti-A2BP1 / Fox1 antibody images

  • All lanes : Anti-A2BP1 / Fox1 antibody (ab94581) at 1 µg/ml

    Lane 1 : Forebrain (Mouse) Tissue Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 110 kDa,28 kDa,37 kDa,56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab94581 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab94581 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa, Hek293, and HepG2 cell types at 1ug/ml (4% formaldehyde fixed, 10 min)

  • IHC image of A2BP1 / Fox1 staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94581, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-A2BP1 / Fox1 antibody (ab94581)

ab94581 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry regarding our two antibodies (ab83574 and ab94581). Generally, brain (CNS) cells express Fox1 at high levels particularly in the following region cerebral cortex, hippocampus and lateral ventricle. As the datasheets indic...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"