A431 nuclear extract lysate (EGF treated) (ab14635)

Overview

  • Product nameA431 nuclear extract lysate (EGF treated)
    See all A431 lysates
  • Description
    A431 nuclear extract lysate
  • General notesCell line: A431 (Human epithelial carcinoma).
    Cells were cultured in medium supplemented with 10 ng/mL EGF (epidermal growth factor) for 10 minutes at 37°C immediately prior to harvesting.
    We recommend aliquoting the extracts into single use fractions and then storing.

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT, 10 ng/mL EGF (epidermal growth factor). pH 7.9
  • Concentration information loading...
  • Research areas
  • BackgroundA431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

References for A431 nuclear extract lysate (EGF treated) (ab14635)

ab14635 has not yet been referenced specifically in any publications.

Product Wall

J'ai mis en place l'envoi d'une unité gratuite de lysat de A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated, référence ab14635. Le numéro de commande est : *****.

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Thank you for your enquiry. ab14635 (A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated) has been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Positive Transcription Factors in Wes...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"