General notesCell line: A431 (Human epithelial carcinoma).
Cells were cultured in medium supplemented with 10 ng/mL EGF (epidermal
growth factor) for 10 minutes at 37°C immediately prior to harvesting.
We recommend aliquoting the extracts into single use fractions and then
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
BackgroundA431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.
References for A431 nuclear extract lysate (EGF treated) (ab14635)
has not yet been referenced specifically in any publications.
Publishing research using ab14635? Please let us know so that we can cite the reference in this datasheet.
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