Overview

  • Product nameAnti-A4GNT antibody [5B5]
    See all A4GNT primary antibodies
  • Description
    Mouse monoclonal [5B5] to A4GNT
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 121-340 of Human A4GNT produced in E.coli (NP_057245).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY A4GNT cDNA; Human pancreas carcinoma tissue.
  • General notesDilute in PBS (pH7,30) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab131649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 39 kDa.
IHC-P 1/150.

Target

  • FunctionNecessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.
  • Tissue specificityDetected in stomach and pancreas.
  • PathwayProtein modification; protein glycosylation.
  • Sequence similaritiesBelongs to the glycosyltransferase 32 family.
  • DomainThe conserved DXD motif is involved in enzyme activity.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4-N-acetylglucosaminyltransferase antibody
    • A4GCT_HUMAN antibody
    • A4GNT antibody
    • Alpha 1 4 N acetylglucosaminyltransferase antibody
    • Alpha-1 antibody
    • Alpha4GnT antibody
    • EC 2.4.1. antibody
    • MGC149493 antibody
    see all

Anti-A4GNT antibody [5B5] images

  • All lanes : Anti-A4GNT antibody [5B5] (ab131649)

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY A4GNT cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 39 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC221256)
  • Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labelling A4GNT with ab131649 at 1/150 dilution.

References for Anti-A4GNT antibody [5B5] (ab131649)

ab131649 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"