Overview

  • Product name
    Anti-A4GNT antibody
  • Description
    Goat polyclonal to A4GNT
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-PRTYRDLIKGPEGS

    , corresponding to C terminal amino acids 317-330 of Human A4GNT (NP_057245.1).

  • Positive control
    • K562 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).

Target

  • Function
    Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.
  • Tissue specificity
    Detected in stomach and pancreas.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 32 family.
  • Domain
    The conserved DXD motif is involved in enzyme activity.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4-N-acetylglucosaminyltransferase antibody
    • A4GCT_HUMAN antibody
    • A4GNT antibody
    • Alpha 1 4 N acetylglucosaminyltransferase antibody
    • Alpha-1 antibody
    • Alpha4GnT antibody
    • EC 2.4.1. antibody
    • MGC149493 antibody
    see all

Images

  • Anti-A4GNT antibody (ab99187) at 1 µg/ml + K562 cell lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

References

ab99187 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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