Overview

  • Product nameAnti-AAK1 antibodySee all AAK1 primary antibodies ...
  • Description
    Rabbit polyclonal to AAK1
  • SpecificityThis antibody detects endogenous levels of total AAK1 protein.
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic peptide derived from the internal region of human AAK1

  • Positive control
    • Extracts from COS7 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab66005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 94, 75 kDa (predicted molecular weight: 94 kDa).

Target

  • FunctionPhosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationMembrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAK 1 antibody
    • Aak1 antibody
    • AAK1_HUMAN antibody
    • Adaptor associated kinase 1 antibody
    • Adaptor-associated kinase 1 antibody
    • AP2 associated kinase 1 antibody
    • AP2 associated protein kinase 1 antibody
    • AP2-associated protein kinase 1 antibody
    • DKFZp686F03202 antibody
    • DKFZp686K16132 antibody
    • FLJ23712 antibody
    • FLJ25931 antibody
    • FLJ31060 antibody
    • FLJ42882 antibody
    • FLJ45252 antibody
    • KIAA1048 antibody
    • MGC138170 antibody
    • MGC164568 antibody
    • MGC164570 antibody
    see all

Anti-AAK1 antibody images

  • All lanes : Anti-AAK1 antibody (ab66005) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells
    Lane 2 : Extracts from COS7 cells, plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 94 kDa
    Observed band size : 94 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-AAK1 antibody (ab66005)

ab66005 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"