Overview

  • Product nameAnti-AAK1 antibody
    See all AAK1 primary antibodies
  • Description
    Rabbit polyclonal to AAK1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic 20 amino acid peptide from near the amino terminus of human AAK1 (NP_055726).

  • Positive control
    • Rat spleen lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 94 kDa.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • FunctionPhosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationMembrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAK 1 antibody
    • Aak1 antibody
    • AAK1_HUMAN antibody
    • Adaptor associated kinase 1 antibody
    • Adaptor-associated kinase 1 antibody
    • AP2 associated kinase 1 antibody
    • AP2 associated protein kinase 1 antibody
    • AP2-associated protein kinase 1 antibody
    • DKFZp686F03202 antibody
    • DKFZp686K16132 antibody
    • FLJ23712 antibody
    • FLJ25931 antibody
    • FLJ31060 antibody
    • FLJ42882 antibody
    • FLJ45252 antibody
    • KIAA1048 antibody
    • MGC138170 antibody
    • MGC164568 antibody
    • MGC164570 antibody
    see all

Anti-AAK1 antibody images

  • Lane 1 : Anti-AAK1 antibody (ab77082) at 1 µg/ml
    Lane 2 : Anti-AAK1 antibody (ab77082) at 2 µg/ml

    Lane 1 : A-20 lysate
    Lane 2 : A-20 lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 94 kDa
    Observed band size : 94 kDa

References for Anti-AAK1 antibody (ab77082)

ab77082 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your call today and for your question about ab77082.

I have heard back from the lab, and though the exact immunogen sequence is considered proprietary, it does fall between amino acids 181 and 240. This region is within the cata...

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