Overview

  • Product name
  • Description
    Rabbit polyclonal to AARE
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Pig, Common marmoset
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human AARE.

    (Peptide available as ab90215.)

  • Positive control
    • This antibody gave a positive signal in human liver tissue lysate and the following whole cell lysates: HepG2; HeLa; Jurkat; HEK293; MOLT4; Caco2.

Properties

Applications

Our Abpromise guarantee covers the use of ab80538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).

Target

  • Function
    This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
  • Sequence similarities
    Belongs to the peptidase S9C family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AARE antibody
    • ACPH antibody
    • ACPH_HUMAN antibody
    • Acyl peptide hydrolase antibody
    • Acyl-peptide hydrolase antibody
    • Acylamino acid releasing enzyme antibody
    • Acylamino-acid-releasing enzyme antibody
    • Acylaminoacyl peptidase antibody
    • Acylaminoacyl-peptidase antibody
    • APEH antibody
    • APH antibody
    • N acylaminoacyl peptide hydrolase antibody
    • OPH antibody
    • Oxidized protein hydrolase antibody
    see all

Images

  • All lanes : Anti-AARE antibody (ab80538) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 7 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 81 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 61 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab80538 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80538, 20µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 oat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 10µg/ml, and in 100% Methanol fixed (5 min) Hek293 cells at 10µg/ml.

References

ab80538 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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