The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
FunctionCatalyzes the post-translational modification of target proteins by phosphopantetheine. Can transfer the 4'-phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, such as the acyl carrier domain of FASN.
Tissue specificityDetected in heart, skeletal muscle, placenta, testis, brain, pancreas, liver and kidney.
Sequence similaritiesBelongs to the P-Pant transferase superfamily. AcpS family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.