Overview

  • Product nameAnti-AATF antibody
    See all AATF primary antibodies
  • Description
    Rabbit polyclonal to AATF
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 12 amino acid synthetic peptide from near the carboxy terminus of human AATF.

  • Positive control
    • Human small intestine cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab39631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • FunctionMay function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
  • Tissue specificityUbiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
  • Sequence similaritiesBelongs to the AATF family.
  • Post-translational
    modifications
    Hyperphosphorylated during the G1/S phase transition.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AATF antibody
    • AATF_HUMAN antibody
    • Apoptosis antagonizing transcription factor antibody
    • Apoptosis-antagonizing transcription factor antibody
    • CHE 1 antibody
    • CHE1 antibody
    • DED antibody
    • Protein AATF antibody
    • Rb binding protein Che 1 antibody
    • Rb-binding protein Che-1 antibody
    see all

Anti-AATF antibody images

  • Lane 1 : Anti-AATF antibody (ab39631) at 0.5 µg/ml
    Lane 2 : Anti-AATF antibody (ab39631) at 1 µg/ml
    Lane 3 : Anti-AATF antibody (ab39631) at 2 µg/ml

    Lane 1 : Human small intestine cell lysate
    Lane 2 : Human small intestine cell lysate
    Lane 3 : Human small intestine cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    anti-rabbit IgG HRP at 1/10000 dilution

    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    (Lanes read from left to right, 1, 2, 3.)
  • ab39631 at 10µg/ml staining AATF in small intestine by IHC

  • Immunofluorescence of AATF in Human Small Intestine cells using ab39631 at 20 ug/ml.

References for Anti-AATF antibody (ab39631)

This product has been referenced in:

See 1 Publication for this product

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