• Product name
  • Description
    Rabbit polyclonal to AATF
  • Specificity
    ab65248 detects endogenous levels of total AATF protein.
  • Tested applications
    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from N terminus of human AATF.

  • Positive control
    • Extracts from HeLa cells, HepG2 cells, COLO205 cells and HUVEC cells.



Our Abpromise guarantee covers the use of ab65248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).


  • Function
    May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
  • Tissue specificity
    Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
  • Sequence similarities
    Belongs to the AATF family.
  • Post-translational
    Hyperphosphorylated during the G1/S phase transition.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AATF antibody
    • AATF_HUMAN antibody
    • Apoptosis antagonizing transcription factor antibody
    • Apoptosis-antagonizing transcription factor antibody
    • BFR2 antibody
    • CHE 1 antibody
    • CHE1 antibody
    • DED antibody
    • Protein AATF antibody
    • Rb binding protein Che 1 antibody
    • Rb-binding protein Che-1 antibody
    see all


  • All lanes : Anti-AATF antibody (ab65248) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HepG2 cell extract
    Lane 3 : COLO205 cell extract
    Lane 4 : HUVEC cell extract
    Lane 5 : HUVEC cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • ICC/IF image of ab65248 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65248, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab65248 has not yet been referenced specifically in any publications.

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