The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
Use at 2-5 µg/mg of lysate.
FunctionMay function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Tissue specificityUbiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Sequence similaritiesBelongs to the AATF family.
Post-translational modificationsHyperphosphorylated during the G1/S phase transition.
Detection of Human AATF by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72148 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
References for Anti-AATF antibody (ab72148)
has not yet been referenced specifically in any publications.
Publishing research using ab72148? Please let us know so that we can cite the reference in this datasheet.
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