Overview

  • Product nameAnti-AATF antibody
    See all AATF primary antibodies
  • Description
    Rabbit polyclonal to AATF
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 125 and 175 of human AATF (NP_036270.1).

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionMay function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
  • Tissue specificityUbiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
  • Sequence similaritiesBelongs to the AATF family.
  • Post-translational
    modifications
    Hyperphosphorylated during the G1/S phase transition.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AATF antibody
    • AATF_HUMAN antibody
    • Apoptosis antagonizing transcription factor antibody
    • Apoptosis-antagonizing transcription factor antibody
    • CHE 1 antibody
    • CHE1 antibody
    • DED antibody
    • Protein AATF antibody
    • Rb binding protein Che 1 antibody
    • Rb-binding protein Che-1 antibody
    see all

Anti-AATF antibody images

  • All lanes : Anti-AATF antibody (ab72148) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 62 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Detection of Human AATF by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72148 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

References for Anti-AATF antibody (ab72148)

ab72148 has not yet been referenced specifically in any publications.

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