You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameFas Human ELISA KitSee all CD95 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 5 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 92.28 83% - 105%
    Tissue Extracts 89.79 82% - 104%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100514 Fas Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Fas in cell lysate and tissue lysate. This assay employs an antibody specific for human Fas coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Fas antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • FunctionReceptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • Tissue specificityIsoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • Involvement in diseaseDefects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • Sequence similaritiesContains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • DomainContains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession P25445 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        ALPS1AAPO 1APO 1 cell surface antigen
        Apo-1 antigenAPO1Apo1 antigenApoptosis antigen 1Apoptosis mediating surface antigen FASApoptosis-mediating surface antigen FASAPT1 CD95CD95 antigenFasFas AMAFas antigenFAS1FASLG receptorFASTM TNF receptor superfamily member 6TNFRSF6 TNR6_HUMANTumor necrosis factor receptor superfamily member 6
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100514 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Fas Human ELISA Kit images

      • ELISA - CD95 Human ELISA Kit (ab100514)
        ELISA - CD95 Human ELISA Kit (ab100514)

      Protocols

      References for Fas Human ELISA Kit (ab100514)

      ab100514 has not yet been referenced specifically in any publications.

      Publishing research using ab100514 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit a Question for ab100514

      There are currently no Questions for ab100514.
      Please use the link above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"