Overview
- Product nameFGF9 Human ELISA KitSee all FGF9 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 200 pg/ml
- Range358.4 pg/ml - 35000 pg/ml
- Recovery
97 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 100.27 88% - 107% Serum 97.52 85% - 105% Plasma 94.38 83% - 102% - Species reactivityReacts with: Human
- Product overview
FGF9 (Fibroblast growth factor 9) is a member of the fibroblast growth factor (FGF) family of proteins. Human FGF9 is a protein of 208 amino acids with sequence similarity of approximately 30 percent to other members of the family of FGF. FGF9 is found in the conditioned medium of a Human glioma cell line and acts on cells of the central nervous system. It is a potent mitogen for glial cells.
ab100520 FGF9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF9 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human FGF9 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human FGF9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Notes
Optimization may be required with urine samples
- Tested applicationsELISA more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 300X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A diluent buffer, For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody FGF-9: biotinylated anti-human FGF-9 2 vials FGF-9 Microplate (12 strips x 8 wells) coated with anti-human FGF-9 1 unit FGF-9 Standard Human Recombinant 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml -
Research Areas
modificationsThree molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
N-glycosylated.
Target information above from: UniProt accession
P31371
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Elbow knee synostosisFGF 9FGF-9
- FGF9FGF9_HUMANFibroblast growth factor 9GAFGlia Activating Factor Glia-activating factorHBFG 9 HBFG9HBGF-9Heparin binding growth factor 9Heparin-binding growth factor 9MGC119914 MGC119915SYNS3
see all
- Entrez Gene: 2254 Human
- Omim: 600921 Human
- SwissProt: P31371 Human
- Unigene: 111 Human
Applications
Our Abpromise guarantee covers the use of ab100520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
FGF9 Human ELISA Kit images
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ELISA - FGF9 Human ELISA Kit (ab100520)
Protocols
References for FGF9 Human ELISA Kit (ab100520)
ab100520 has not yet been referenced specifically in any publications.
