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Overview

  • Product nameMMP3 Human ELISA KitSee all MMP3 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 0.3 ng/ml
  • Range
    0.41 ng/ml - 100 ng/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.72 84% - 103%
    Serum 95.39 83% - 103%
    Plasma 96.25 85% - 104%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100607 MMP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP3 pro and active forms in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended) and cell culture supernatants.

    This assay employs an antibody specific for human MMP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion tothe amount of MMP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • Involvement in diseaseDefects in MMP3 are the cause of susceptibility to coronary heart disease type 6 (CHDS6) [MIM:614466]. A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries. Note=A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P08254 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CHDS6Matrix metalloproteinase 3 Matrix metalloproteinase 3 preproprotein
        Matrix metalloproteinase-3MGC126102MGC126103MGC126104MMP 3MMP-3MMP3MMP3_HUMANProgelatinaseProteoglycanaseSL 1 SL-1SL1STMYSTMY1STR1 Stromelisin 1 Stromelysin 1Stromelysin 1 progelatinase Stromelysin-1Transin 1Transin-1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100607 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      MMP3 Human ELISA Kit images

      • ELISA - MMP3 Human ELISA Kit (ab100607)
        ELISA - MMP3 Human ELISA Kit (ab100607)
      • ELISA - MMP3 Human ELISA Kit (ab100607)
        ELISA - MMP3 Human ELISA Kit (ab100607)Image courtesy of an anonymous Abreview.
        The graph shows the differing levels of MMP3 released by human primary fibroblasts (200µg) exposed to several different active ingredients dissolved in media for several days prior to media collection and analysis.

        See Abreview

      Protocols

      References for MMP3 Human ELISA Kit (ab100607)

      ab100607 has not yet been referenced specifically in any publications.

      Publishing research using ab100607 ? Please let us know so that we can cite the reference in this datasheet

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      Abreview for MMP3 Human ELISA Kit

       Good Good 4/5 (Ease of Use)
      Abreviews

      Sample: Human Cell (Skin fibroblasts)
      Specification: Skin fibroblasts
      Amount of Sample: 200 µg
      Type of Kit Used: ELISA
      Positive control: Included in kit as part of standard curve.
      Did you use the Abcam Kit protocol?: Yes

      Additional data
      Additional Notes: Incubation times are a little longer than in some other kits, which must be remembered when planning multiple experiments, but the data generated is of a better quality overall.

      The graph shows the differing levels of MMP3 released by primary fibroblasts exposed to several different active ingredients dissolved in media for several days prior to media collection and analysis.

      (Sorry I cant say what the actives actually are due to business nature of the work).
      abreview image
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      Verified customer

      Submitted Feb 09 2012

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"