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Overview

  • Product nameTREM1 Human ELISA KitSee all TREM1 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 70 pg/ml
  • Range
    0.069 ng/ml - 50 ng/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 124.4 106% - 134%
    Serum 125.5 91% - 145%
    Plasma 116 94% - 115%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100659 Human TREM1 (triggering receptor expressed on myeloid cells 1) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TREM1 in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human TREM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TREM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TREM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TREM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • RelevanceTREM1 is also known as triggering receptor expressed on myeloid cells 1, and triggering receptor expressed on monocytes 1. TREM1 is a Type I membrane protein that contains an immunoglobulin-like V-type domain. An alternatively spliced protein variant may be secreted. TREM1 is highly expressed on peripheral blood myeloid cells (particularly mature monocytes and granulocytes); TREM1 expression can be further upregulated by bacteria, fungi and lipopolysaccharide. TREM1 has been shown to interact with the adaptor protein DAP12 to stimulate neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines. TREM1 is thought to amplify inflammatory responses to fungal and bacterial infections and potentiate septic shock.
  • Cellular localizationSecreted and Cell membrane.
    • Alternative names
        CD354TREM 1Triggering receptor expressed on monocytes 1
        Triggering receptor expressed on myeloid cells 1Triggering receptor TREM 1Triggering receptor TREM1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100659 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      TREM1 Human ELISA Kit images

      • ELISA - TREM1 Human ELISA Kit (ab100659)
        ELISA - TREM1 Human ELISA Kit (ab100659)

      Protocols

      References for TREM1 Human ELISA Kit (ab100659)

      ab100659 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"