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Overview

  • Product nameEotaxin-2 (CCL24) Mouse ELISA KitSee all Eotaxin 2 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 4 pg/ml
  • Range
    2.46 pg/ml - 600 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.65 89% - 102%
    Serum 103.1 93% - 112%
    Plasma 113.1 79% - 137%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    The ab100681 Mouse Eotaxin 2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Eotaxin 2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse Eotaxin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Eotaxin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceEotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.
  • Cellular localizationSecreted
    • Alternative names
        C C motif chemokine 24CCL24Chemokine (C C motif) ligand 24
        Chemokine CC Motif Ligand 2CK beta 6Ckb 6Ckb6Eosinophil chemotactic protein 2Eotaxin 2 like proteinMPIF 2MPIF2 Myeloid progenitor inhibitory factor 2 OTTHUMP00000210225SCYA24Small inducible cytokine A24Small inducible cytokine subfamily A (Cys Cys) member 24
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100681 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Eotaxin-2 (CCL24) Mouse ELISA Kit images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab100681

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab100681

      Protocols

      References for Eotaxin-2 (CCL24) Mouse ELISA Kit (ab100681)

      ab100681 has not yet been referenced specifically in any publications.

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