HGF Mouse ELISA Kit (ab100686)
- Product nameHGF Mouse ELISA KitSee all HGF kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 100 pg/ml
- Range0.25 ng/ml - 60 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 111.8 98% - 123% Serum 111.4 98% - 129% Plasma 105.8 80% - 120%
- Species reactivityReacts with: Mouse
- Product overview
ab100686 Mouse HGF (Hepatocyte Growth Factor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse HGF in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Kit is for the quantitative measurement of mouse HGF in serum, plasma and cell culture supernatants.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 500X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A diluent buffer . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium) diluent. 1 x 15ml Detection Antibody HGF : biotinylated anti-mouse HGF 2 vials HGF Microplate (12 strips x 8 wells) coated with anti-mouse HGF 1 unit HGF Standard Mouse (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain.
- DFNB39F TCFFibroblast derived tumor cytotoxic factor
- Hepapoietin AHepatocyte growth factorHepatocyte growth factor (hepapoietin A scatter factorHepatocyte growth factor beta chainHepatopoietin-AHgfHGF_HUMANHGFBHPTALung fibroblast derived mitogenScatter factorSF
Our Abpromise guarantee covers the use of ab100686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for HGF Mouse ELISA Kit (ab100686)
ab100686 has not yet been referenced specifically in any publications.