Mouse IGF1 ELISA Kit (ab100695)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Tissue Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IGF1 ELISA Kit
See all IGF1 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma, Tissue Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 4 pg/ml -
Range
2.74 pg/ml - 2000 pg/ml -
Recovery
98 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 86.4 72% - 99% Serum 101.2 85% - 117% Plasma 106.6 85% - 114% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s IGF1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IGF1 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse IGF1 2 vials IGF1 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IGF1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IBP1
- IGF I
- IGF IA
see all -
Database links
- Entrez Gene: 16000 Mouse
- SwissProt: P05017 Mouse
- Unigene: 268521 Mouse
Images
Datasheets and documents
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SDS download
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Datasheet download
References (31)
ab100695 has been referenced in 31 publications.
- Thyne KM & Salmon AB Metabolic benefits of methionine restriction in adult mice do not require functional methionine sulfoxide reductase A (MsrA). Sci Rep 12:5073 (2022). PubMed: 35332198
- Hill CM et al. FGF21 is required for protein restriction to extend lifespan and improve metabolic health in male mice. Nat Commun 13:1897 (2022). PubMed: 35393401
- Recinella L et al. Protective effects of growth hormone-releasing hormone analogs in DSS-induced colitis in mice. Sci Rep 11:2530 (2021). PubMed: 33510215
- Young K et al. Decline in IGF1 in the bone marrow microenvironment initiates hematopoietic stem cell aging. Cell Stem Cell 28:1473-1482.e7 (2021). PubMed: 33848471
- Minnis-Lyons SE et al. Notch-IGF1 signaling during liver regeneration drives biliary epithelial cell expansion and inhibits hepatocyte differentiation. Sci Signal 14:N/A (2021). PubMed: 34158399