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Overview

  • Product nameLeptin Mouse ELISA KitSee all Leptin kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 4 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.47 83% - 103%
    Serum 93.29 82% - 102%
    Plasma 95.38 84% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Leptin is a secreted protein of 16 kDa. Human and Murine leptin show approximately 84 percent identity at the protein level. Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Studies indicate that Human obesity may be associated with leptin receptor deficiencies rather than constituting a problem with leptin itself.

    ab100718 Mouse Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Leptin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Mouse Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Leptin is a secreted protein of 16 kDa. Human and Murine leptin show approximately 84 percent identity at the protein level. Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Studies indicate that Human obesity may be associated with leptin receptor deficiencies rather than constituting a problem with leptin itself.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the leptin family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P41159 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        FLJ94114LEPLEP_HUMAN
        LeptinLeptin Murine Obesity Homolog Leptin Precursor Obesity FactorOBObese proteinObesity factorObesity homolog mouse Obesity murine homolog leptinOBS
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100718 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Leptin Mouse ELISA Kit images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab100718

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab100718

      Protocols

      References for Leptin Mouse ELISA Kit (ab100718)

      ab100718 has not yet been referenced specifically in any publications.

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