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Overview

  • Product nameLeptin Rat ELISA KitSee all Leptin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 30 pg/ml
  • Range
    10.97 pg/ml - 8000 pg/ml
  • Recovery

    84 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.43 68% - 89%
    Serum 92.32 83% - 102%
    Plasma 84.25 75% - 94%

  • Species reactivity
    Reacts with: Rat
  • Product overview

    ab100773 Rat Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Leptin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Rat Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    200X HRP-Streptavidin Concentrate Item G 1 x 200µl
    20X Wash Buffer Concentrate Item B 1 x 25ml
    Assay Diluent B 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent. 1 x 15ml
    Assay Diluent D 5x concentrated buffer. For Standard/Sample (serum/plasma samples/cell culture medium/urine) diluent. 1 x 15ml
    Detection Antibody Leptin: biotinylated anti-rat Leptin 2 vials
    Leptin Microplate (12 strips x 8 wells) coated with anti-rat Leptin 1 unit
    Leptin Standard Rat (recombinant) 2 vials
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the leptin family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P41159 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        FLJ94114LEPLEP_HUMAN
        LeptinLeptin Murine Obesity Homolog Leptin Precursor Obesity FactorOBObese proteinObesity factorObesity homolog mouse Obesity murine homolog leptinOBS
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100773 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Leptin Rat ELISA Kit images

      • ELISA - Leptin Rat ELISA Kit (ab100773)
        ELISA - Leptin Rat ELISA Kit (ab100773)

      Protocols

      References for Leptin Rat ELISA Kit (ab100773)

      ab100773 has not yet been referenced specifically in any publications.

      Publishing research using ab100773 ? Please let us know so that we can cite the reference in this datasheet

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      Abreviews

      Sample: Rat Serum (Blood)
      Specification: Blood
      Amount of Sample: 50 µg
      Type of Kit Used: ELISA
      Positive control: rat serum (diabetic)
      Did you use the Abcam Kit protocol?: Yes
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      Submitted May 07 2012

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"