Leptin Rat ELISA Kit (ab100773)
- Product nameLeptin Rat ELISA KitSee all Leptin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 30 pg/ml
- Range10.97 pg/ml - 8000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 75.43 68% - 89% Serum 92.32 83% - 102% Plasma 84.25 75% - 94%
- Species reactivityReacts with: Rat
- Product overview
ab100773 Rat Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Leptin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Rat Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent B 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent. 1 x 15ml Assay Diluent D 5x concentrated buffer. For Standard/Sample (serum/plasma samples/cell culture medium/urine) diluent. 1 x 15ml Detection Antibody Leptin: biotinylated anti-rat Leptin 2 vials Leptin Microplate (12 strips x 8 wells) coated with anti-rat Leptin 1 unit Leptin Standard Rat (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- LeptinLeptin Murine Obesity Homolog Leptin Precursor Obesity FactorOBObese proteinObesity factorObesity homolog mouse Obesity murine homolog leptinOBS
Our Abpromise guarantee covers the use of ab100773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Leptin Rat ELISA Kit (ab100773)
ab100773 has not yet been referenced specifically in any publications.