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Overview

  • Product nameVEGF Rat ELISA KitSee all VEGF kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 2 pg/ml
  • Range
    0.82 pg/ml - 200 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 92.67 81% - 102%
    Tissue Extracts 94.34 82% - 102%

  • Species reactivity
    Reacts with: Rat
  • Product overview

    ab100787 VEGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat VEGF cell lysate and tissue lysate. This assay employs an antibody specific for rat VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    2X Cell Lysate Buffer Item J 1 x 5ml
    Assay Diluent 5x concentrated buffer. For Detection Antibody and HRP-Streptavidin concentrate diluent. 1 x 15ml
    Detection Antibody VEGF: biotinylated anti-rat VEGF 2 vials
    HRP-Streptavidin Concentrate 120x concentrated HRP-conjugated streptavidin 1 x 200µl
    Sample Diluent Buffer 5x concentrated buffer For Standard/Sample (cell lysate/tissue lysate) diluent. 1 x 10ml
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
    VEGF Microplate (12 strips x 8 wells) coated with anti-rat VEGF 1 unit
    VEGF Standard Rat (recombinant) 2 vials
  • Research Areas
  • RelevanceVEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated.
    • Alternative names
        MGC70609MVCD1Vascular endothelial growth factor
        Vascular endothelial growth factor AVascular permeability factorVEGF AVEGFAVPF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100787 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      VEGF Rat ELISA Kit images

      • ELISA - VEGF Rat ELISA Kit (ab100787)
        ELISA - VEGF Rat ELISA Kit (ab100787)

      Protocols

      References for VEGF Rat ELISA Kit (ab100787)

      ab100787 has not yet been referenced specifically in any publications.

      Publishing research using ab100787 ? Please let us know so that we can cite the reference in this datasheet

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      Submit a Question for ab100787

      Displaying 1 - 3 of 3 results for Q&A

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      We are using abcam VEGF rat ELISA Kit(ab100787) to assay VEGF concentration in DRG tissues. This kit works well. But, when we measured the tissue lysate protein concentration by using BCA protein assay  kit, it is no work. Please give me some advices, how...

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      The BCA total protein assay will be fine with our lysis buffer. In fact, we use it all the time in our lab to determine the total protein in lysate samples. To my knowledge, the Bradford assay is the only total protein assay which is http://en.wikipedia.o...

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      What is the concentration of the streptavidin-HRP reagent provided with this kit?

      Thank you for contacting us.

      The concentration of the HRP-streptavidin reagent is considered proprietary information by the developer of the kit. I am sorry we could not be of more help.

      Please do not hesitate to contact us if you ha...

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      The new kits work fine. We think we found the problem - original kit shipped to us had manual printed in 2010 that called for diluting HRP 1:6000; new kit shipped to us has manual printed in 2012 that calls for diluting HRP 1:120.

      I am happy to hear that the new kits are working well for you! Please let me know if there is anything else I can do to help.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"