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Overview

  • Product nameCaspase Cascade Screening KitSee all Caspase kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    0h 30m
  • Product overview

    Abcam's Caspase Cascade Screening Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D2R), a reported substrate for members of caspase family proteases. The caspase substrate D2R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of members of caspase-family proteases plays a key role in apoptosis.

  • Tested applicationsFunctional Studies, Flow Cyt more details

Properties

  • RelevanceCaspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
  • Cellular localizationCytoplasmic

    • Applications

    Our Abpromise guarantee covers the use of ab102499 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS
    Flow Cyt Flow Cyt

    Protocols

    References for Caspase Cascade Screening Kit (ab102499)

    ab102499 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"