Caspase Cascade Screening Kit (ab102499)
- Product nameCaspase Cascade Screening KitSee all Caspase kits ...
- Tests100 x 1 assay
- Sample typeAdherent cells, Suspension cells
- Assay typeDirect
- Assay time0h 30m
- Product overview
Abcam's Caspase Cascade Screening Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D2R), a reported substrate for members of caspase family proteases. The caspase substrate D2R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.
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Activation of members of caspase-family proteases plays a key role in apoptosis.
- Tested applicationsFunctional Studies, Flow Cyt more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests D2R Incubation Buffer 1 x 30ml D2R Reagent 1 x 100µl DTT 1 x 300µl
- Research Areas
Our Abpromise guarantee covers the use of ab102499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Flow Cyt|
References for Caspase Cascade Screening Kit (ab102499)
ab102499 has not yet been referenced specifically in any publications.