Aspartate Assay Kit (ab102512)
- Product nameAspartate Assay Kit
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeQuantitative
- Sensitivity> 2 µM
- Range2 µM - 200 µM
- Assay time0h 40m
- Product overview
Abcam's Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 µM).
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L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys). Aspartate is a metabolite in the urea cycle, participates in gluconeogenesis and transports reducing equivalents between the cytosol and the mitochondria via the malate-aspartate shuttle. Aspartate also stimulates NMDA receptors (not as strongly as glutamate) and hence serves as an excitatory neurotransmitter in the brain and is an excitotoxin.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Aspartate Assay Buffer — Aspartate Enzyme Mix — Aspartate Probe (in DMSO solution) — Aspartate Standard — Conversion Mix — Serum CleanUp Mix —
- Research Areas
Our Abpromise guarantee covers the use of ab102512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Aspartate Assay Kit images
Colorimetric Standard Curve Performed as Described in the Protocol
Fluorometric Standard Curve Performed as Described in the Protocol
References for Aspartate Assay Kit (ab102512)
ab102512 has not yet been referenced specifically in any publications.