Overview
- Product nameAspartate Assay Kit
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeQuantitative
- Sensitivity> 2 µM
- Range2 µM - 200 µM
- Assay time0h 40m
- Product overview
Abcam's Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 µM).
Visit our FAQs page for tips and troubleshooting. - Notes
L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys). Aspartate is a metabolite in the urea cycle, participates in gluconeogenesis and transports reducing equivalents between the cytosol and the mitochondria via the malate-aspartate shuttle. Aspartate also stimulates NMDA receptors (not as strongly as glutamate) and hence serves as an excitatory neurotransmitter in the brain and is an excitotoxin.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 100 tests Aspartate Assay Buffer — Aspartate Enzyme Mix — Aspartate Probe (in DMSO solution) — Aspartate Standard — Conversion Mix — Serum CleanUp Mix — - Research Areas
Applications
Our Abpromise guarantee covers the use of ab102512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Aspartate Assay Kit images
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Functional Studies - Aspartate Assay Kit (ab102512)Colorimetric Standard Curve Performed as Described in the Protocol -
Functional Studies - Aspartate Assay Kit (ab102512)Fluorometric Standard Curve Performed as Described in the Protocol
Protocols
References for Aspartate Assay Kit (ab102512)
ab102512 has not yet been referenced specifically in any publications.
