Overview
- Product nameAnti-GRP94 antibodySee all GRP94 primary antibodies ...
- DescriptionRabbit polyclonal to GRP94
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 20-50 of Human GRP94.
- Positive controlK562 cell line and Mouse liver tissue lysates; Human lung carcinoma tissue.
Properties
- FormLiquid
- Storage instructionsStore at 4°C (up to 6 months). For long term storage store at -20°C
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab104039 is purified through a protein A column, followed by peptide affinity purification.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab104039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/100 - 1/500. Predicted molecular weight: 92 kDa. |
| IHC-P | IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- FunctionMolecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
- Sequence similaritiesBelongs to the heat shock protein 90 family.
- Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
-
Database links
- Entrez Gene: 7184 Human
- Entrez Gene: 22027 Mouse
- Omim: 191175 Human
- SwissProt: P14625 Human
- SwissProt: P08113 Mouse
- Unigene: 192374 Human
- Unigene: 87773 Mouse
Target information above from: UniProt accession
P14625
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- 94 kDa glucose regulated protein antibody94 kDa glucose-regulated protein antibodyECGP antibody
- Endoplasmin antibodyEndothelial cell (HBMEC) glycoprotein antibodyENPL_HUMAN antibodyGlucose regulated protein 94kDa antibodygp96 antibodygp96 homolog antibodyGRP 94 antibodyGRP-94 antibodyHeat shock protein 90 kDa beta member 1 antibodyHSP90B1 antibodyStress inducible tumor rejection antigen GP96 antibodyTRA1 antibodyTumor rejection antigen 1 antibodyTumor rejection antigen gp96 antibody
see all
Anti-GRP94 antibody images
-
Western blot - GRP94 antibody (ab104039)Anti-GRP94 antibody (ab104039) at 1/100 dilution + K562 cell line lysates at 35 µg
Predicted band size : 92 kDa -
Western blot - GRP94 antibody (ab104039)Anti-GRP94 antibody (ab104039) at 1/100 dilution + Mouse liver tissue lysates at 35 µg
Predicted band size : 92 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP94 antibody (ab104039)ab104039 at 1/50 dilution, staining GRP94 in Human lung carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
References for Anti-GRP94 antibody (ab104039)
ab104039 has not yet been referenced specifically in any publications.
