Anti-Plzf antibody [5B3] (ab104854)

Overview

• Product nameAnti-Plzf antibody [5B3]See all Plzf primary antibodies ...
• Description
  Mouse monoclonal [5B3] to Plzf
• Tested applicationsELISA, WB, ICC/IF, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Purified recombinant fragment of Human Plzf expressed in E. Coli.

• Positive controlHela cell lysate. Hela cells

Properties

• FormLiquid
• Storage instructionsStore at 4°C. Aliquot and store at -20°C long-term.
• Storage bufferPreservative: 0.03% Sodium Azide
  Constituents: Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number5B3
• IsotypeIgG1
• Research Areas
  • Developmental Biology
  • Reproduction
  • Germ cell markers

  • Stem Cells
  • Hematopoietic Progenitors
  • Intracellular Molecules

  • Stem Cells
  • Germline Stem Cells
  • Embryonic Germ Cells

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Transcription factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

Applications

Target

• FunctionProbable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
• Tissue specificityWithin the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.
• PathwayProtein modification; protein ubiquitination.
• Involvement in diseaseDefects in ZBTB16 are the cause of skeletal defects genital hypoplasia and mental retardation (SGYMR) [MIM:612447]. A disorder characterized by mental retardation, craniofacial dysmorphism, microcephaly and short stature. Additional features include absence of the thumbs, hypoplasia of the radii and ulnae, additional vertebrae and ribs, retarded bone age and genital hypoplasia.
  Note=A chromosomal aberration involving ZBTB16 may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with RARA.
• Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
  Contains 1 BTB (POZ) domain.
  Contains 9 C2H2-type zinc fingers.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q05516 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7704 Human
  • Omim: 176797 Human
  • SwissProt: Q05516 Human
  • Unigene: 591945 Human
  • Unigene: 682144 Human

• Alternative names
  AI467657 antibodylu antibodyPLZF antibody

  Promyelocytic leukemia zinc finger protein antibodyZBT16_HUMAN antibodyZBTB16 antibodyZfp145 antibodyZinc finger and BTB domain containing 16 antibodyzinc finger and BTB domain containing protein 16 antibodyZinc finger and BTB domain-containing protein 16 antibodyZinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia) antibodyZinc finger protein 145 antibodyZinc finger protein PLZF antibodyZnf145 antibody

  see all

Anti-Plzf antibody [5B3] images

• 

  Western blot - Plzf antibody [5B3] (ab104854)
  Anti-Plzf antibody [5B3] (ab104854) at 1/500 dilution + HeLa cell lysate
  
  Predicted band size : 74 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Plzf antibody [5B3] (ab104854)
  Immunofluorescence analysis of Hela cells using ab104854 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
• 

  Flow Cytometry - Anti-Plzf antibody [5B3] (ab104854)
  Overlay histogram showing K562 cells stained with ab104854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.