Overview
- Product nameAnti-TGF beta 1 antibody [9016.2]See all TGF beta 1 primary antibodies ...
- DescriptionMouse monoclonal [9016.2] to TGF beta 1
- SpecificityBy ELISA, the antibody shows less than 5% crossreactivity with TGF beta 1.2 and less than 1% cross-reactivity with TGF beta 2, TGF beta 3 and TGF beta 5.
- Tested applicationsELISA, Inhibition Assay, WB more details
- Species reactivityReacts with: Human
- Immunogen
Recombinant full length protein (Human) expressed in CHO cells.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS, 0.25M Sodium chloride, 0.01M Sodium phosphate -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- Clone number9016.2
- IsotypeIgG1
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab10517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | |
| Inhibition Assay | |
| WB |
ELISA: Use at a concentration of 2 - 4 µg/ml. Determined for 9 pg/ml of recombinant human TGF beta 1.
Inhib: Use at a concentration of 0.3 - 1 µg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
- Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
- Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
- Sequence similaritiesBelongs to the TGF-beta family.
- Post-translational
modificationsGlycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. - Cellular localizationSecreted > extracellular space > extracellular matrix.
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Database links
- Entrez Gene: 7040 Human
- Omim: 190180 Human
- SwissProt: P01137 Human
- Unigene: 645227 Human
Target information above from: UniProt accession
P01137
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Camurati Engelmann disease antibodyCED antibodyDiaphyseal dysplasia 1 progressive antibody
- DPD1 antibodyLAP antibodyLatency-associated peptide antibodyTGF beta 1 antibodyTGF beta antibodyTGF beta 1 protein antibodyTGF-beta 1 protein antibodyTGF-beta-1 antibodyTGF-beta-5 antibodyTGF-beta1 antibodyTGFB 1 antibodyTGFB antibodyTgfb-1 antibodyTGFB1 antibodyTGFB1_HUMAN antibodyTGFbeta antibodyTGFbeta1 antibodyTransforming Growth Factor b1 antibodyTransforming Growth Factor beta 1 antibodyTransforming growth factor beta 1a antibodytransforming growth factor beta-1 antibodytransforming growth factor, beta 1 antibody
see all
References for Anti-TGF beta 1 antibody [9016.2] (ab10517)
This product has been referenced in:
- Soller EC et al. Common features of optimal collagen scaffolds that disrupt wound contraction and enhance regeneration both in peripheral nerves and in skin. Biomaterials 33:4783-91 (2012). WB ; Rat . Read more (PubMed: 22483241) »
- Wang L et al. Concentration-dependent effects of transforming growth factor ß1 on corneal wound healing. Mol Vis 17:2835-46 (2011). Inhibition Assay ; Human . Read more (PubMed: 22128231) »
