Overview
- Product nameAnti-IL1 beta antibody (HRP)See all IL1 beta primary antibodies ...
- DescriptionRabbit polyclonal to IL1 beta (HRP)
- ConjugationHRP
- Specificityab106035 is primarily directed against mature, 17,000 MW Mouse IL1 beta. It will recognize 10% of the non-denatured (native) precursor 31,000 MW Mouse IL1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. ab106035 does not detect IL1 alpha.
- Tested applicationsWB, IHC-P, Flow Cyt, IP, RIA, IHC-Fr, ELISA, Neutralising more details
- Species reactivityReacts with: Mouse, Rat
Does not react with
Human, Non Human Primates - Immunogen
Recombinant Mouse IL1 beta produced in E.coli. The MW of recombinant mouse IL1 beta was 17 kDa
- Positive controlRat and Mouse endotoxin stimulated peripheral blood mononuclear cells (PBMC).
- General notesDo NOT add Sodium Azide!
Properties
- FormLiquid
- Storage instructionsStore at 4°C. Aliquot and store at -20°C long-term.
- Storage bufferpH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride, 1% BSA
Note: BSA is Immunoglobulin and Protease free -
Concentration information loading... - PurityDEAE-Chromatography
- Purification notesab106035 was heated to 56° C for 30 minutes.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab106035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000 - 1/5000. Predicted molecular weight: 17, 30 kDa. |
| IHC-P | IHC-P: 1/500 - 1/2500. |
| Flow Cyt | Flow Cyt: Use at an assay dependent concentration. Caution should be exhibited as the F(c) domain of the Rabbit IgG molecule may interact with cells non-specifically. |
| IP | IP: Use at an assay dependent concentration. Preclearing the preparation with a non-specific Rabbit IgG to reduce background is suggested. |
| RIA | RIA: Use at an assay dependent concentration. |
| IHC-Fr | IHC-Fr: 1/500 - 1/2500. |
| ELISA | ELISA: 1/10000 - 1/50000. Best used as the second antibody in combination with a monoclonal antibody as a capture antibody. |
| Neutralising | Neut: Use at an assay dependent concentration. It is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested. |
Target
- FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
- Sequence similaritiesBelongs to the IL-1 family.
- DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
- Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
-
Database links
- Entrez Gene: 16176 Mouse
- Entrez Gene: 24494 Rat
- SwissProt: P10749 Mouse
- SwissProt: Q63264 Rat
- Unigene: 222830 Mouse
- Unigene: 9869 Rat
Target information above from: UniProt accession
P01584
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- Catabolin antibodyH1 antibodyIL 1 antibody
- IL 1 beta antibodyIL-1 beta antibodyIL1 BETA antibodyIL1B antibodyIL1B_HUMAN antibodyIL1F2 antibodyInterleukin 1 beta antibodyInterleukin-1 beta antibodyOAF antibodyOTTHUMP00000162031 antibodyPreinterleukin 1 beta antibodyPro interleukin 1 beta antibody
see all
References for Anti-IL1 beta antibody (HRP) (ab106035)
ab106035 has not yet been referenced specifically in any publications.
