Anti-5-Methyl Cytidine antibody [33D3] - ChIP Grade (ab10805)

Overview

• Product nameAnti-5-Methyl Cytidine antibody [33D3] - ChIP GradeSee all 5-Methyl Cytidine primary antibodies ...
• Description
  Mouse monoclonal [33D3] to 5-Methyl Cytidine - ChIP Grade
• SpecificityClone 33D3 has been developed to discriminate between the modified base 5-MeCyd and the normal counterpart cytosine.
• Tested applicationsIHC-P, MeDIP, ChIP, IP, WB, Southern Blot, Flow Cyt, IHC-Fr more details
• Immunogen

  Recognises the modified base 5-methylcytidine found in DNA of plants and vertebrates.

• General notesStorage in frost-free freezers is not recommended. Should this product contain a precipitate microcentrifugation before use.
  
  While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunately, we can no longer guarantee this antibody for use in ICC.

  
  

  Ab10805 recognises the modified base 5-methylcytidine found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal
• Concentration information loading...
• PurityIgG fraction
• Primary antibody notes Ab10805 recognises the modified base 5-methylcytidine found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.
• Clonality Monoclonal
• Clone number33D3
• MyelomaSp2/0-Ag14
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA / Nucleotides

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • DNA methylation
  • Methylated DNA

Applications

Target

• RelevanceCytidine is a nucleoside formed by a cytosine attached to a ribose ring via a ß-N1-glycosidic bond. DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), a potent epigenetics marker and regulator of gene expression. The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), formyl- (5-fC), and carboxy- (5-caC) cytidine. Several enzymes, including the Tet family of proteins have been implicated in cytidine demethylation.
• Cellular localizationNuclear

• Alternative names
  5 m C antibody5 mC antibody5 me C antibody

  5 Me Cytidine antibody5 Me Cytosine antibody5 meC antibody5 MeCyd antibody5 Methyl Cytidine antibody5 Methyl Cytosine antibody5 MethylCytidine antibody5 MethylCytosine antibody5-mC antibody5-Me Cytidine antibody5-Me Cytosine antibody5-meC antibody5-Methyl Cytidine antibody5-Methyl Cytosine antibody5-Methyl-Cytidine antibody5-Methyl-Cytosine antibody5-MethylCytidine antibody5-MethylCytosine antibody5mC antibody

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Anti-5-Methyl Cytidine antibody [33D3] - ChIP Grade images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 5-Methyl Cytidine antibody [ 33D3] (ab10805)This image is courtesy of an Abreview submitted by Ms Sara Maj Wätjen Hyldig

  ab10805 staining 5-Methyl Cytidine in pig embryo (15 to 17 days) tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Tris-EDTA buffer, permeabilization in Triton X-100 and blocking in 2% BSA for 10 minutes at 25°C. The primary antibody was diluted, 1/100 (PBS + 2% BSA) and incubated with sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse at 1/250 dilution, was used as secondary.

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