DLL1 protein (ab108549)
Our Abpromise guarantee covers the use of ab108549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||FuncS: Use at an assay dependent dilution.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
- DeltaDelta (Drosophila) like 1Delta like 1
- Delta like 1 (Drosophila)Delta like 1 proteinDelta like protein 1Delta like protein 1 precursorDelta, Drosophila, homolog of 1Delta-like protein 1Delta1DL1DLL 1DLL1DLL1_HUMANDrosophila Delta homolog 1H-Delta-1OTTHUMP00000017690
Contains 8 EGF-like domains.
modificationsUbiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
DLL1 protein images
Adipogenesis inhibition. Macroscopic pictures.
Adipogenesis inhibition of 3T3L1 cells.
3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1µM Dexamethasone, 0.5mM IBMX, 10µg/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5µg/ml mDLL1-Fc (ab108549) and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol.
Induction of Hes-1 with the treatment of recombinant mouse DLL1-Fc (ab108549).
A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5mg/ml of mouse DLL1-Fc (ab108549) as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Lane 1: ab108549, 0 min
Lane 2: ab108549, 10 min
Lane 3: ab108549, 30 min
Lane 4: ab108549, 1 hr
Lane 5: ab108549, 2 hr
Lane 6: ab108549, 4 hr
Lane 7: ab108549, 8 hr
Lane 8: ab108549, 24 hr
References for DLL1 protein (ab108549)
ab108549 has not yet been referenced specifically in any publications.