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Overview

  • Product nameDLL1 proteinSee all DLL1 proteins and peptides ...
  • Protein descriptionRecombinant fragment corresponding to amino acids 1-545 (signal peptide and extracellular domain) of Mouse DLL1 fused at the C-terminus to the Fc portion of Human IgG1, MWt 100 kDa (by SDS-PAGE) (UniProt Q61483).
  • Uniprot accessionQ61483
  • Molecular weight100kDa inclusive of tags
  • Protein length545 amino acids
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesab108549 is 0.2µm filtered.
  • Biological activityInhibits adipogenesis of 3T3L1 cells. Induces Hes1 in 3T3L1 cells. Interacts with Notch receptors.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • Additional notesWorking aliquots are stable for up to 3 months when stored at -20°C. Inhibits adipogenesis of 3T3L1 cells. Induces Hes1 in 3T3L1 cells. Interacts with Notch receptors.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab108549 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        DeltaDelta (Drosophila) like 1Delta like 1
        Delta like 1 (Drosophila)Delta like 1 proteinDelta like protein 1Delta like protein 1 precursorDelta, Drosophila, homolog of 1Delta-like protein 1Delta1DL1DLL 1DLL1DLL1_HUMANDrosophila Delta homolog 1H-Delta-1OTTHUMP00000017690
      see all
  • FunctionActs as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • Tissue specificityExpressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • Sequence similaritiesContains 1 DSL domain.
    Contains 8 EGF-like domains.
  • Post-translational
    modifications    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession O00548 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    DLL1 protein images

    • Functional Studies - DLL1 protein (ab108549)
      Functional Studies - DLL1 protein (ab108549)
      Adipogenesis inhibition. Macroscopic pictures.
    • Functional Studies - DLL1 protein (ab108549)
      Functional Studies - DLL1 protein (ab108549)
      Adipogenesis inhibition of 3T3L1 cells.
      3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1µM Dexamethasone, 0.5mM IBMX, 10µg/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5µg/ml mDLL1-Fc (ab108549) and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol.
    • Functional Studies - DLL1 protein (ab108549)
      Functional Studies - DLL1 protein (ab108549)
      Induction of Hes-1 with the treatment of recombinant mouse DLL1-Fc (ab108549).
      A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5mg/ml of mouse DLL1-Fc (ab108549) as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

      Lane 1: ab108549, 0 min
      Lane 2: ab108549, 10 min
      Lane 3: ab108549, 30 min
      Lane 4: ab108549, 1 hr
      Lane 5: ab108549, 2 hr
      Lane 6: ab108549, 4 hr
      Lane 7: ab108549, 8 hr
      Lane 8: ab108549, 24 hr

    References for DLL1 protein (ab108549)

    ab108549 has not yet been referenced specifically in any publications.

    Publishing research using ab108549 ? Please let us know so that we can cite the reference in this datasheet

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