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Chorionic Gonadotropin beta Human ELISA Kit (ab108638)

Overview

  • Product nameChorionic Gonadotropin beta Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Serum
  • Assay typeSandwich
  • Sensitivity
    = 0.25 ng/ml
  • Range
    0.25 ng/ml - 50 ng/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab108638, Chorionic Gonadotropin Beta Human ELISA Kit is intended for the quantitative research of Chorionic Gonadotropin Beta in human serum.

    Human Chorionic Gonadotropin (hCG) is a glycoprotein hormone normally produced by placenta during pregnancy. The hormone is present in blood and urine around seven to thirteen days following implantation of the fertilized ovum. Structurally intact hCG molecules consist of two non-covalently linked polypeptide subunits, the alpha and beta chain subunits. Measurement of intact hCG and of the alpha subunit of hCG appears to give similar results in blood and urine but not the levels of beta subunit. In the normal second-trimester maternal sera, the level of intact hCG range from 20,000 mIU/ml to 50,000 mIU/ml (1 ng = 15 mIU). In contrast, the levels of either free alpha or free beta-hCG are on average one half of 1% of hCG levels. hCG and the free subunits appear not to be useful as serological markers for nontrophoblastic tumors; however, the absolute increase of beta-hCG level in choriocarcinoma patients clearly differentiates it from normal pregnancy.

     

  • Tested applicationsELISA, Sandwich ELISA more details
  • PlatformMicroplate

Properties

  • Alternative names
      CG-betaCGBCGB3
      CGB5CGB7CGB8Choriogonadotropin subunit betaChorionic gonadotrophin chain betahCG
    see all
  • Database links
  • Applications

    Our Abpromise guarantee covers the use of ab108638 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA
    Sandwich ELISA sELISA

    Chorionic Gonadotropin beta Human ELISA Kit images

    Protocols

    References for Chorionic Gonadotropin beta Human ELISA Kit (ab108638)

    ab108638 has not yet been referenced specifically in any publications.

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    Abreviews

    Sample: Human Recombinant protein (Culture media collected from human colorectal cell)
    Specification: Culture media collected from human colorectal cell
    Amount of Sample: .002-.050 µg
    Type of Kit Used: ELISA
    Positive control: Spiked fresh culture media and mouse urine with protein provided for standard curves
    Did you use the Abcam Kit protocol?: Yes
    Username

    Verified customer

    Submitted Mar 11 2013

    Abreviews

    Sample: Human Cell (Epithelial ovarian cancer)
    Specification: Epithelial ovarian cancer
    Amount of Sample: 20 µg
    Type of Kit Used: ELISA
    Positive control: Conditioned media from BEWO cells which are known to secrete Chorionic Gonadotropin beta.
    Did you use the Abcam Kit protocol?: Yes

    Additional data
    Additional Notes: Chorionic Gonadotropin beta could be detected from conditioned media without having to concentrate the media. However, if collecting media from a few cells, a longer incubation time or concentrating the media may be necessary.
    Username

    Verified customer

    Submitted May 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"