Apolipoprotein AII Human ELISA Kit (ab108805)
Overview
- Product nameApolipoprotein AII Human ELISA Kit
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Milk, Urine, Serum, Plasma, Cell culture extracts
- Assay typeSandwich
- Sensitivity= 0.004 µg/ml
- Range0.004 µg/ml - 4 µg/ml
- Recovery
97 %
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Apolipoprotein A-II (ApoA-II) is the second most abundant apolipoproteins in human plasma HDL, comprising about 25% of the protein mass. After being synthesized by the liver and intestine as a preprotein containing 100 amino acids, ApoA-II is processed to 77 amino acids in the mature plasma protein. ApoA-II is found in plasma as a monomer, homodimer of 17.4 kDa, or heterodimer with ApoE and ApoD. It has been reported that ApoA-II plays roles in HDL remodeling, cholesterol efflux, modulating HDL interaction with enzymes and receptors, triglyceride metabolism, and atherosclerosis. ApoA-II is inversely associated with risk of future coronary artery disease. Serum levels of an isoform of ApoA-II have been identified as a potential marker for prostate cancer.
Ab108805 Human ApoA-II ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human ApoA-II in plasma, serum, urine and cell culture supernatant. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human ApoA-II in less than 4 hours. A monoclonal antibody specific for human ApoA-II has been pre-coated onto a 96-well microplate with removable strips. ApoA-II in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for human ApoA-II, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- PlatformMicroplate
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Biotinylated ApoA-II Antibody 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Apo A-II Microplate 1 x 96 units Human ApoA-II Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml - Research Areas
- SwissProt: P02652 Human
Applications
Our Abpromise guarantee covers the use of ab108805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
Apolipoprotein AII Human ELISA Kit images
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ELISA - Apolipoprotein AII Human ELISA Kit (ab108805)
Protocols
References for Apolipoprotein AII Human ELISA Kit (ab108805)
ab108805 has not yet been referenced specifically in any publications.
