Ferritin Human ELISA KIt (ab108837)
- Product nameFerritin Human ELISA KItSee all Ferritin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Milk, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 1.5 ng/ml
- Range1.563 ng/ml - 50 ng/ml
- Assay time4h 0m
- Species reactivityReacts with: Human
Predicted to work with: Monkey
- Product overview
Ferritin is an iron storage protein. It consists of 24 subunits with combined molecular weight of 474,000. Serum Ferritin level is related to body iron stores and is influenced by several diseases. High serum Ferritin levels associate with iron overload, diabetes mellitus, Adult-onset Still disease (AOSD), excessive macrophage activation and alcohol intake. On the other hand, low levels of Ferritin may indicate Iron Deficiency Anemia.
ab108837 Ferritin Human ELISA kit is designed for detection of Human Ferritin in plasma, serum, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures Ferritin in less than 4 hours. A polyclonal antibody specific for Ferritin has been pre-coated onto a microplate. Ferritin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Ferritin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Ferritin Antibody (100x) 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Ferritin Microplate (12 strips of 8 wells) 1 unit Ferritin Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
Contains 1 ferritin-like diiron domain.
- ferritin L chainFerritin L subunitFerritin light chain
- ferritin light polypeptideferritin light polypeptide like 3FRIL_HUMANFTLNBIA3
Our Abpromise guarantee covers the use of ab108837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Ferritin Human ELISA KIt (ab108837)
ab108837 has not yet been referenced specifically in any publications.