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Overview

  • Product nameFerritin Human ELISA KItSee all Ferritin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Milk, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.563 ng/ml - 50 ng/ml
  • Recovery

    97 %

  • Assay time
    4h 0m
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Monkey
  • Product overview

    Ferritin is an iron storage protein. It consists of 24 subunits with combined molecular weight of 474,000. Serum Ferritin level is related to body iron stores and is influenced by several diseases. High serum Ferritin levels associate with iron overload, diabetes mellitus, Adult-onset Still disease (AOSD), excessive macrophage activation and alcohol intake. On the other hand, low levels of Ferritin may indicate Iron Deficiency Anemia.

    ab108837 Ferritin Human ELISA kit is designed for detection of Human Ferritin in plasma, serum, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures Ferritin in less than 4 hours. A polyclonal antibody specific for Ferritin has been pre-coated onto a microplate. Ferritin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Ferritin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionStores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • Involvement in diseaseDefects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • Sequence similaritiesBelongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.

    • Target information above from: UniProt accession P02792 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        ferritin L chainFerritin L subunitFerritin light chain
        ferritin light polypeptideferritin light polypeptide like 3FRIL_HUMANFTLNBIA3
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108837 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Ferritin Human ELISA KIt images

      • ELISA - Ferritin Human ELISA KIt (ab108837)
        ELISA - Ferritin Human ELISA KIt (ab108837)
        Example standard curve

      Protocols

      References for Ferritin Human ELISA KIt (ab108837)

      ab108837 has not yet been referenced specifically in any publications.

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