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Overview

  • Product nameFibronectin Mouse ELISA kitSee all Fibronectin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 3 ng/ml
  • Range
    3.13 ng/ml - 200 ng/ml
  • Recovery

    97 %

  • Assay time
    3h 30m
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Fibronectin (FN) is a major component of the extracellular matrix and blood plasma, and is a specific ligand for several integrin adhesion receptors. FN plays an important role not only in cell adhesion and wound healing, but also in embryogenesis and hematopoiesis. FN is over-expressed in cardiovascular disease states such as atherosclerosis and myocardial infarction. Reduced levels of FN have been reported in patients with Disseminated Intravascular Coagulation (DIC) and low concentrations appear to correlate with a poor prognosis.


    ab108849 Fibronectin Mouse ELISA kit is designed for detection of Mouse fibronectin in plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures fibronectin in 3.5 hours. A polyclonal antibody specific for fibronectin has been pre-coated onto a microplate. Fibronectin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for fibronectin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • RelevanceFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. Interacts with FBLN7 By similarity. Interacts with COMP. Interacts with S.aureus fnbA. Interacts with TNR; the interaction inhibits cell adhesion and neurite outgrowth By similarity. Interacts with FST3. Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. Sulfated. It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylation sites are present in the extracelllular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2) [MIM:601894]; also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Contains 12 fibronectin type-I domains. Contains 2 fibronectin type-II domains. Contains 16 fibronectin type-III domains.
  • Cellular localizationSecreted, extracellular space, extracellular matrix
    • Alternative names
        CIGCold insoluble globulinFibronectin 1
        FINCFNFN1FNZMigration stimulating factorMSF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108849 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Fibronectin Mouse ELISA kit images

      • ELISA - Fibronectin Mouse ELISA kit (ab108849)
        ELISA - Fibronectin Mouse ELISA kit (ab108849)
        Standard curve

        The curve provided is for illustration only. A standard curve should be generated each time the assay is performed.

      Protocols

      References for Fibronectin Mouse ELISA kit (ab108849)

      This product has been referenced in:
      • Riopel MM  et al. ß1 integrin-extracellular matrix interactions are essential for maintaining exocrine pancreas architecture and function. Lab Invest N/A:N/A (2012). Read more (PubMed: 23069938) »

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