You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameHemopexin Human ELISA KitSee all Hemopexin kits ...
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 0.07 µg/ml
  • Range
    0.078 µg/ml - 5 µg/ml
  • Recovery

    99.5 %

  • Assay time
    2h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Hemopexin is a heme-binding plasma glycoprotein which, after haptoglobin, forms the second line of defense against hemoglobin-mediated oxidative damage during intravascular hemolysis. A decrease in plasma Hemopexin concentration reflects a recent release of heme compounds in the extracellular compartment. Heme–Hemopexin complexes are delivered to hepatocytes by receptor-mediated endocytosis after which Hemopexin is recycled to the circulation. Studies indicated that increased Hemopexin level associate with minimal change disease (MCD), sporadic Alzheimer's disease (AD), heavy-drinking chronic alcoholics, hemolytic anemias, chronic neuromuscular diseases and acute intermittent porphyria.

    ab108859 Hemopexin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Hemopexin in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures Human Hemopexin in less than 2 hours. A polyclonal antibody specific for Human Hemopexin has been pre-coated onto a 96-well microplate with removable strips. Hemopexin in standards and samples is competed by a biotinylated hemopexin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsCompetitive ELISA, ELISA more details

Properties

  • RelevanceBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • Cellular localizationSecreted
    • Alternative names
        beta-1B-glycoproteinHPXHpxnHX
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108859 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Competitive ELISA cELISA
      ELISA ELISA

      Hemopexin Human ELISA Kit images

      • Competitive ELISA - Hemopexin Human ELISA Kit (ab108859)
        Competitive ELISA - Hemopexin Human ELISA Kit (ab108859)
        Human Hemopexin Standard Curve

      Protocols

      References for Hemopexin Human ELISA Kit (ab108859)

      ab108859 has not yet been referenced specifically in any publications.

      Publishing research using ab108859 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108859

      Filter by Ratings

      Displaying 1 - 1 of 1 results for Abreviews

      10
      Votes: Highest First

      Abreview for Hemopexin Human ELISA Kit

       Excellent Excellent 5/5 (Ease of Use)
      Abreviews

      Sample: Human Serum
      Amount of Sample: 2 ul µg
      Type of Kit Used: ELISA
      Did you use the Abcam Kit protocol?: Yes

      Additional data
      Additional Notes: We diluted 2ul of plasma with 800 ul of Diluent (1:400).
      We washed the plate 6 times with a machine.
      The standards data generated an excelent curve with a fitting error of
      3.48e-5.
      All samples assayed showed data inside the curve.
      abreview image
      Username

      Verified customer

      Submitted Mar 26 2012

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"