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Overview

  • Product nameHemopexin Human ELISA KitSee all Hemopexin kits ...
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 0.07 µg/ml
  • Range
    0.078 µg/ml - 5 µg/ml
  • Recovery

    99.5 %

  • Assay time
    2h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Hemopexin is a heme-binding plasma glycoprotein which, after haptoglobin, forms the second line of defense against hemoglobin-mediated oxidative damage during intravascular hemolysis. A decrease in plasma Hemopexin concentration reflects a recent release of heme compounds in the extracellular compartment. Heme–Hemopexin complexes are delivered to hepatocytes by receptor-mediated endocytosis after which Hemopexin is recycled to the circulation. Studies indicated that increased Hemopexin level associate with minimal change disease (MCD), sporadic Alzheimer's disease (AD), heavy-drinking chronic alcoholics, hemolytic anemias, chronic neuromuscular diseases and acute intermittent porphyria.

    ab108859 Hemopexin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Hemopexin in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures Human Hemopexin in less than 2 hours. A polyclonal antibody specific for Human Hemopexin has been pre-coated onto a 96-well microplate with removable strips. Hemopexin in standards and samples is competed by a biotinylated hemopexin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsCompetitive ELISA, ELISA more details

Properties

  • RelevanceBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • Cellular localizationSecreted
    • Alternative names
        beta-1B-glycoproteinHPXHpxnHX
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108859 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Competitive ELISA cELISA
      ELISA ELISA

      Hemopexin Human ELISA Kit images

      • Competitive ELISA - Hemopexin Human ELISA Kit (ab108859)
        Competitive ELISA - Hemopexin Human ELISA Kit (ab108859)
        Human Hemopexin Standard Curve

      Protocols

      References for Hemopexin Human ELISA Kit (ab108859)

      ab108859 has not yet been referenced specifically in any publications.

      Publishing research using ab108859 ? Please let us know so that we can cite the reference in this datasheet

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      Submit a Question for ab108859

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      What are the immunogens for the antibodies in these kits?

      Thank you for your patience with this reply.

      I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

      1) Apolipoprotein A1 Human ELISA Kit
      2) Ap...

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      I recently purchased a Human Hemopexin ELISA kit #ab108859 from your company and I am confused by the directions for diluting the lyophilized standard. The directions in the protocol say to dilute with appropriate amount of diluents to make a standard sol...

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      Thank you for your enquiry.

      Sorry for the confusion here. A large excess of Standard is provided in this ELISA kit. To properly dilute the standard, just reconstitute the 3.5ug Standard you received in 0.7ml of Diluent to produce a 5ug/ml Standa...

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      Can you please send me data using this kit, which shows values obtained for serum or plasma samples.

      Thank you for your phone call. Please find testing data for ab108859 attached. I hope this helps, please let me know if you need any additional information or assistance.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"