Hemopexin Human ELISA Kit (ab108859)
- Product nameHemopexin Human ELISA KitSee all Hemopexin kits ...
- Sample typeSerum, Plasma
- Assay typeCompetitive
- Sensitivity= 0.07 µg/ml
- Range0.078 µg/ml - 5 µg/ml
- Assay time2h 00m
- Species reactivityReacts with: Human
- Product overview
Hemopexin is a heme-binding plasma glycoprotein which, after haptoglobin, forms the second line of defense against hemoglobin-mediated oxidative damage during intravascular hemolysis. A decrease in plasma Hemopexin concentration reflects a recent release of heme compounds in the extracellular compartment. Heme–Hemopexin complexes are delivered to hepatocytes by receptor-mediated endocytosis after which Hemopexin is recycled to the circulation. Studies indicated that increased Hemopexin level associate with minimal change disease (MCD), sporadic Alzheimer's disease (AD), heavy-drinking chronic alcoholics, hemolytic anemias, chronic neuromuscular diseases and acute intermittent porphyria.
ab108859 Hemopexin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Hemopexin in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures Human Hemopexin in less than 2 hours. A polyclonal antibody specific for Human Hemopexin has been pre-coated onto a 96-well microplate with removable strips. Hemopexin in standards and samples is competed by a biotinylated hemopexin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsCompetitive ELISA, ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Hemopexin 1 unit Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Hemopexin Microplate 1 unit Hemopexin Standard 1 x 5µg Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml
- Research Areas
Our Abpromise guarantee covers the use of ab108859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Hemopexin Human ELISA Kit (ab108859)
ab108859 has not yet been referenced specifically in any publications.