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alpha 2 Macroglobulin Human ELISA Kit (ab108883)

Abreviews (1)Q&A (2)

Overview

  • Product namealpha 2 Macroglobulin Human ELISA KitSee all alpha 2 Macroglobulin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk
  • Assay typeSandwich
  • Sensitivity
    = 2 ng/ml
  • Range
    0.002 µg/ml - 0.5 µg/ml
  • Recovery

    97.5 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Alpha 2 Macroglobulin is a major serum protein with diverse functions, including inhibition of protease activity and binding of growth factors, cytokines, and disease factors. Increased serum alpha 2 Macroglobulin has been suggested to be associated with multiple sclerosis (MS), glomerular disease, and with liver diseases.

    ab108883 is designed for detection of Human alpha 2 Macroglobulin in saliva, milk and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures alpha 2 Macroglobulin in 4 hours. A polyclonal antibody specific for alpha 2 Macroglobulin has been pre-coated onto a microplate. Alpha 2 Macroglobulin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for alpha 2 Macroglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsELISA, Sandwich ELISA more details

Properties

  • FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • Tissue specificitySecreted in plasma.
  • Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P01023 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5A2mA2MD
        A2MG_HUMANAlpha-2-MAlpha-2-macroglobulinC3 and PZP-like alpha-2-macroglobulin domain-containing protein 5CPAMD5FWP007Pregnancy zone proteinS863-7
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108883 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA
      Sandwich ELISA sELISA

      alpha 2 Macroglobulin Human ELISA Kit images

      • ELISA - alpha 2 Macroglobulin Human ELISA Kit
        ELISA - alpha 2 Macroglobulin Human ELISA Kit
        ELISA analysis of alpha 2 Macroglobulin in HepG2 cell conditioned media and fresh growth media containing FBS, using ab108883 alpha 2 Macroglobulin Human ELISA Kit. This image is courtesy of an anonymous Abreview.

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      • Sandwich ELISA - alpha 2 Macroglobulin Human ELISA Kit - 1 x 96 wells plate (ab108883)
        Sandwich ELISA - alpha 2 Macroglobulin Human ELISA Kit - 1 x 96 wells plate (ab108883)

      Protocols

      References for alpha 2 Macroglobulin Human ELISA Kit (ab108883)

      ab108883 has not yet been referenced specifically in any publications.

      Publishing research using ab108883 ? Please let us know so that we can cite the reference in this datasheet

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      Abreviews

      Sample: Human Cell (HepG2)
      Specification: HepG2
      Amount of Sample: 0.125ug standard µg
      Type of Kit Used: ELISA
      Positive control: Provided alpha 2 Macroglobulin standard
      Did you use the Abcam Kit protocol?: Yes

      Additional data
      Additional Notes: Recognized human A2M. Did not recognize bovine A2M in FBS.
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      Submitted Jul 03 2012

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"