alpha Macroglobulin Mouse ELISA Kit (ab108886)
Overview
- Product namealpha Macroglobulin Mouse ELISA KitSee all alpha Macroglobulin kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeCompetitive
- Sensitivity= 1.5 µg/ml
- Range1.56 µg/ml - 100 µg/ml
- Recovery
95 %
- Assay time3h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Mouse
- Product overview
Alpha 2 Macroglobulin is a major serum protein with diverse functions, including inhibition of protease activity and binding of growth factors, cytokines, and disease factors. Increased serum alpha 2 Macroglobulin has been suggested to be associated with multiple sclerosis (MS), glomerular disease, and with liver diseases.
Mouse alpha Macroglobulin is believed to be a functional homologue of human alpha 2 Macroglobulin.
ab108886 is designed for detection of mouse alpha Macroglobulin in plasma, and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures mouse alpha Macroglobulin in less than 3 hours. A polyclonal antibody specific for mouse alpha Macroglobulin has been pre-coated onto a 96-well microplate with removable strips. Alpha Macroglobulin in standards and samples is competed by a biotinylated alpha Macroglobulin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsCompetitive ELISA more details
- PlatformMicroplate
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Alpha Macroglobulin Microplate: 1 unit Alpha Macroglobulin Standard: 1 unit Biotinylated Alpha Macroglobulin: 1 unit Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml - Research Areas
- SwissProt: Q61838 Mouse
Applications
Our Abpromise guarantee covers the use of ab108886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Competitive ELISA | cELISA |
alpha Macroglobulin Mouse ELISA Kit images
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Competitive ELISA - alpha Macroglobulin Mouse ELISA Kit (ab108886)
Protocols
References for alpha Macroglobulin Mouse ELISA Kit (ab108886)
ab108886 has not yet been referenced specifically in any publications.
