Tissue Factor Human ELISA Kit (ab108903)
- Product nameTissue Factor Human ELISA KitSee all Tissue Factor kits ...
- Tests1 x 96 well plate
- Sample typeSerum, Plasma, Cell culture extracts, Tissue
- Assay typeSandwich
- Sensitivity= 20 pg/ml
- Range12.5 pg/ml - 400 pg/ml
- Assay time5h 00m
- Species reactivityReacts with: Human
- Product overview
The transmembrane protein Tissue Factor is the physiologic trigger of coagulation in normal hemostasis. Tissue Factor binds and allosterically activates factor VII. The Tissue factor-VIIa complex cleaves factor IX and X, leading to thrombin generation. Inducible expression of Tissue Factor in a variety of pathological conditions, including gram-negative sepsis and acute coronary syndromes, is associated with life-threatening thrombosis. In sepsis, Tissue Factor expression within the vasculature leads to disseminated intravascular coagulation. Tissue Factor also plays important roles in vasculogenesis, metastasis, and tumor-associated angiogenesis.
ab108903 Tissue Factor Human ELISA kit is designed for detection of Human Tissue Factor in plasma, serum, tissue and cell culture lysate. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Tissue Factor in less than 5 hours. A polyclonal antibody specific for Tissue Factor has been pre-coated onto a 96-well microplate. Tissue Factor in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for Tissue Factor, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
This assay recognizes both natural and recombinant Human Tissue Factor apoprotein and Tissue factor/FVII complexes.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Tissue Factor Antibody (100x) 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Tissue Factor Microplate 1 x 96 units Tissue Factor Standard 1 unit Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
- CD142Coagulation factor IIIF3
Our Abpromise guarantee covers the use of ab108903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Tissue Factor Human ELISA Kit (ab108903)
ab108903 has not yet been referenced specifically in any publications.